Hisano S, Chikamori-Aoyama M, Katoh S, Kagotani Y, Daikoku S, Chihara K
Department of Anatomy, School of Medicine, University of Tokushima, Japan.
Neurosci Lett. 1988 May 26;88(2):145-50. doi: 10.1016/0304-3940(88)90116-4.
An electron microscopic study showed by using a dual immunolabeling technique that in the suprachiasmatic nucleus of the rat, axon terminals immunoreactive for neuropeptide Y (NPY) made synaptic contacts upon neurons immunoreactive for vasoactive intestinal polypeptide (VIP). Diaminobenzidine (DAB)-labeled NPY axon terminals made synaptic contacts on silver-gold-labeled VIP perikarya and dendritic processes. The presynaptic NPY terminals contained many small clear vesicles and a few cored vesicles labeled with DAB chromogen. At the synaptic portion, a symmetrical thickening of the pre- and post-synaptic membranes was evident.
一项电子显微镜研究通过使用双重免疫标记技术表明，在大鼠的视交叉上核中，对神经肽Y（NPY）免疫反应阳性的轴突终末与对血管活性肠肽（VIP）免疫反应阳性的神经元形成突触联系。二氨基联苯胺（DAB）标记的NPY轴突终末在银金标记的VIP胞体和树突过程上形成突触联系。突触前NPY终末含有许多小而清亮的囊泡以及一些用DAB显色剂标记的有芯囊泡。在突触部位，突触前和突触后膜明显对称增厚。
