Tukmachev V A, Zaslavskiĭ V Iu, Rogozhin S V
Biokhimiia. 1977 Nov;42(11):2058-63.
Effect of a number of homologous series of cationic and anionic surfactants on yeast cells was examined. It is found that the cellular proteins extraction degree under the surfactants treatment depends on the agents structure. It is established that the highest extraction efficiency among the bisquaternary ammonium compounds is displayed by the decyl derivatives and among sodium alkyl sulfates--by octyl sulfate. The influence of pH, ionic strength and the agent concentration on the proteins extraction degree was studied. It is concluded that the anionic and cationic compounds act on the yeast plasma membrane by the similar way and all the differences observed are just the quantitative ones. It is suggested that the efficiency of the surfactant action on the cell is limited particularly by the ratio of cell wall pore size to the size of micelles formed by the agent in the solution.
研究了多种同系物系列的阳离子和阴离子表面活性剂对酵母细胞的影响。发现表面活性剂处理下细胞蛋白质的提取程度取决于试剂的结构。已确定，在双季铵化合物中，癸基衍生物的提取效率最高；在烷基硫酸钠中，硫酸辛酯的提取效率最高。研究了pH值、离子强度和试剂浓度对蛋白质提取程度的影响。得出的结论是，阴离子和阳离子化合物以类似的方式作用于酵母质膜，观察到的所有差异只是数量上的差异。有人提出，表面活性剂对细胞的作用效率尤其受到细胞壁孔径与试剂在溶液中形成的胶束大小之比的限制。
