Shalel Sagit, Streichman Sara, Marmur Abraham
Department of Chemical Engineering, Technion-Israel Institute of Technology, Haifa, 32000, Israel.
J Colloid Interface Sci. 2002 Aug 1;252(1):66-76. doi: 10.1006/jcis.2002.8474.
The effect of ionic strength, solute size, and osmolarity of the suspending solution on surfactant-induced erythrocyte hemolysis was studied. Two possible mechanisms of hemolysis were considered: osmotic lysis (affected by solute particle size) and solubilization (not affected by solute particle size). It was found that the ionic strength of the solution has a major effect on the hemolysis process, depending on the surfactant nature and concentration. An increase in the ionic strength lowers the rate of hemolysis induced by DTAB, and enhances SDS-induced hemolysis. Changes in ionic strength have little effect on hemolysis induced by Triton X-100. To explain these effects, it was assumed that the changes in ionic strength differently affect the adsorption of cationic and anionic surfactants to the membrane. The change in the amount of adsorbed surfactant either influences the rate of osmotic hemolysis by changing the membrane permeability or induces a transition from the osmotic mechanism to solubilization. These phenomena were observed for isotonic as well as hypertonic solutions.
研究了悬浮溶液的离子强度、溶质大小和渗透压对表面活性剂诱导的红细胞溶血的影响。考虑了两种可能的溶血机制：渗透裂解（受溶质颗粒大小影响）和增溶作用（不受溶质颗粒大小影响）。研究发现，溶液的离子强度对溶血过程有主要影响，这取决于表面活性剂的性质和浓度。离子强度的增加降低了由十二烷基三甲基溴化铵（DTAB）诱导的溶血速率，并增强了由十二烷基硫酸钠（SDS）诱导的溶血。离子强度的变化对由曲拉通X-100（Triton X-100）诱导的溶血影响很小。为了解释这些影响，假定离子强度的变化对阳离子和阴离子表面活性剂在膜上的吸附有不同的影响。吸附的表面活性剂数量的变化要么通过改变膜通透性影响渗透溶血速率，要么诱导从渗透机制向增溶作用的转变。在等渗和高渗溶液中均观察到了这些现象。
