Electronic Engineering Department, Ming-Chung University, Taoyuan 33348, Taiwan.
Biotechnology Department, Ming-Chung University, Taoyuan 33348, Taiwan.
Biosensors (Basel). 2021 Mar 18;11(3):86. doi: 10.3390/bios11030086.
Many studies concern the generation of lossy mode resonances (LMRs) using metallic oxide thin films that are deposited on optical fiber. However, the LMR-based optical fiber sensors are frangible, do not allow easy surface modification, and are not suited to mass production. This study proposes an electrical field-induced LMR-based biosensor with an optical planar waveguide to replace surface modification and allow the mass production of protein biosensors and accelerate the speed of the analyte to decrease the detection time. Experimentally, the biosensor is evaluated using charged serum albumin molecules and characterized in terms of the LMR wavelength shift using an externally applied voltage for different durations. The externally applied voltage generates a significant electric field, which drives the non-neutralized biomolecules and increases the LMR wavelength shift. Our experimental results demonstrate that there are two different mechanisms of adsorption of serum albumin molecules for short-term and long-term observations. These are used to calculate the sensitivity of the biosensor. This electrical field-induced method is highly significant for the development and fabrication of LMR-based biosensors.
许多研究都关注使用沉积在光纤上的金属氧化物薄膜来产生损耗模式共振（LMR）。然而，基于 LMR 的光纤传感器易碎，不允许进行简单的表面修饰，不适合大规模生产。本研究提出了一种基于电场诱导的 LMR 的生物传感器，具有光学平面波导，以取代表面修饰并允许大规模生产蛋白质生物传感器，并加速分析物的速度以缩短检测时间。实验中，使用带电的血清白蛋白分子对生物传感器进行了评估，并根据不同持续时间的外部施加电压对 LMR 波长位移进行了特征描述。外部施加的电压会产生显著的电场，从而驱动未中和的生物分子并增加 LMR 波长位移。我们的实验结果表明，对于短期和长期观察，血清白蛋白分子的吸附有两种不同的机制。这些机制被用来计算生物传感器的灵敏度。这种电场诱导方法对于基于 LMR 的生物传感器的开发和制造具有重要意义。
