Yeast Synthetic Biology for the Production of Polysaccharides.

The fruit of L. (goji berry) is used as traditional Chinese medicine, and has the functions of immune regulation, anti-tumor, neuroprotection, anti-diabetes, and anti-fatigue. One of the main bioactive components is polysaccharide (LBP). Nowadays, LBP is widely used in the health market, and it is extracted from the fruit of . The planting of needs large amounts of fields, and it takes one year to harvest the goji berry. The efficiency of natural LBP production is low, and the LBP quality is not the same at different places. Goji berry-derived LBP cannot satisfy the growing market demands. Engineered has been used for the biosynthesis of some plant natural products. Recovery of LBP biosynthetic pathway in and expression of them in engineered might lead to the yeast LBP production. However, information on LBP biosynthetic pathways and the related key enzymes of is still limited. In this review, we summarized current studies about LBP biosynthetic pathway and proposed the strategies to recover key enzymes for LBP biosynthesis. Moreover, the potential application of synthetic biology strategies to produce LBP using engineered was discussed.