Gamble John T, Elson Daniel J, Greenwood Juliet A, Tanguay Robyn L, Kolluri Siva K
Department of Biochemistry & Biophysics, Oregon State University, Corvallis, OR 97331, USA.
Cancer Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA.
Biology (Basel). 2021 Mar 24;10(4):252. doi: 10.3390/biology10040252.
In order to develop new cancer therapeutics, rapid, reliable, and relevant biological models are required to screen and validate drug candidates for both efficacy and safety. In recent years, the zebrafish () has emerged as an excellent model organism suited for these goals. Larval fish or immunocompromised adult fish are used to engraft human cancer cells and serve as a platform for screening potential drug candidates. With zebrafish sharing ~80% of disease-related orthologous genes with humans, they provide a low cost, high-throughput alternative to mouse xenografts that is relevant to human biology. In this review, we provide background on the methods and utility of zebrafish xenograft models in cancer research.
为了开发新的癌症治疗方法，需要快速、可靠且相关的生物学模型来筛选和验证候选药物的疗效和安全性。近年来，斑马鱼已成为适合这些目标的优秀模式生物。幼鱼或免疫受损的成鱼被用于植入人类癌细胞，并作为筛选潜在候选药物的平台。由于斑马鱼与人类共享约80%的疾病相关直系同源基因，它们为与人类生物学相关的小鼠异种移植提供了一种低成本、高通量的替代方法。在本综述中，我们提供了斑马鱼异种移植模型在癌症研究中的方法和应用背景。
