Chindamporn Ariya, Kammarnjessadakul Patcharee, Kesdangsakonwut Sawang, Banlunara Wijit
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Microbiology, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540, Thailand.
Access Microbiol. 2020 Feb 14;2(4):acmi000109. doi: 10.1099/acmi.0.000109. eCollection 2020.
causes pythiosis in humans and animals in tropical and subtropical climates. The clinical manifestations in humans are mostly systemic, vascular or ocular forms, in contrast to animals, which are cutaneous, subcutaneous and gastrointestinal forms. The highest incidence of human cases is reported in Thailand, however, no canine pythiosis has been documented yet.
A female, mixed-breed, stray dog showed severe extensive ulcerative haemorrhagic dermatitis at the perineum involving the anus and tail. On cytology, there were sparse branching septate fungal hyphae. The tissue samples were subjected to polymerase chain reaction and gene sequencing for fungal identification.
The results of the internal transcribed spacer 1 and 2 (ITS1 and ITS2) gene had 99 % homology to (accession no. FJ17396) and the gene (accession no. GQ451572). The phylogenetic tree of both genes was classified in clade A This is the first fully documented diagnosis of canine cutaneous pythiosis in Thailand.
在热带和亚热带气候中可导致人类和动物发生芽生菌病。人类的临床表现大多为全身型、血管型或眼部型，而动物的表现为皮肤型、皮下型和胃肠道型。泰国报告的人类病例发病率最高，然而，尚未有犬芽生菌病的记录。
一只雌性混种流浪狗在会阴处出现严重的广泛性溃疡性出血性皮炎，累及肛门和尾巴。细胞学检查发现有稀疏的分支分隔真菌菌丝。对组织样本进行聚合酶链反应和基因测序以进行真菌鉴定。
内部转录间隔区1和2（ITS1和ITS2）基因的结果与（登录号FJ17396）和基因（登录号GQ451572）有99%的同源性。两个基因的系统发育树被分类在进化枝A中。这是泰国首例有完整记录的犬皮肤芽生菌病诊断病例。
