采用 HS-SPME-GC×GC-TOFMS 技术在尿液中寻找肾小球疾病的潜在标志物。
Searching for Potential Markers of Glomerulopathy in Urine by HS-SPME-GC×GC TOFMS.
机构信息
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Toruń, Poland.
Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 87-100 Toruń, Poland.
出版信息
Molecules. 2021 Mar 24;26(7):1817. doi: 10.3390/molecules26071817.
Volatile organic compounds (VOCs) exiting in urine are potential biomarkers of chronic kidney diseases. Headspace solid phase microextraction (HS-SPME) was applied for extraction VOCs over the urine samples. Volatile metabolites were separated and identified by means of two-dimensional gas chromatography and time of flight mass spectrometry (GC × GC TOF MS). Patients with glomerular diseases ( = 27) and healthy controls ( = 20) were recruited in the study. Different VOCs profiles were obtained from patients and control. Developed methodology offers the opportunity to examine the metabolic profile associated with glomerulopathy. Four compounds found in elevated amounts in the patients group, i.e., methyl hexadecanoate; 9-hexadecen-1-ol; 6,10-dimethyl-5,9-undecadien-2-one and 2-pentanone were proposed as markers of glomerular diseases.
尿液中逸出的挥发性有机化合物（VOCs）是慢性肾脏病的潜在生物标志物。顶空固相微萃取（HS-SPME）被应用于尿液样本中 VOCs 的提取。通过二维气相色谱和飞行时间质谱（GC×GC-TOF-MS）对挥发性代谢物进行分离和鉴定。本研究招募了肾小球疾病患者（=27 例）和健康对照者（=20 例）。从患者和对照组中获得了不同的 VOCs 图谱。所开发的方法为检查与肾小球病相关的代谢谱提供了机会。在患者组中发现了 4 种含量升高的化合物，即甲基十六酸酯；9-十六烯-1-醇；6,10-二甲基-5,9-十一碳二烯-2-酮和 2-戊酮，它们被提议作为肾小球疾病的标志物。
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