Wang Pengcheng, Yang Fangyuan, Ma Zhuo, Zhang Runzhi
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Insects. 2021 Mar 24;12(4):278. doi: 10.3390/insects12040278.
Rice water weevil (RWW) is divided into two types of population, triploid parthenogenesis and diploid bisexual reproduction. In this study, we explored the meiosis of triploid parthenogenesis RWW (Shangzhuang Town, Haidian District, Beijing, China) by marking the chromosomes and microtubules of parthenogenetic RWW oocytes via immunostaining. The immunostaining results show that there is a canonical meiotic spindle formed in the triploid parthenogenetic RWW oocytes, but chromosomes segregate at only one pole, which means that there is a chromosomal unipolar division during the oogenesis of the parthenogenetic RWW. Furthermore, we cloned the conserved sequences of parthenogenetic RWW and , and designed primers based on the parthenogenetic RWW sequence to detect expression patterns by quantitative PCR (Q-PCR). Q-PCR results indicate that the expression of and in ovarian tissue of bisexual is 0.98 and 10,000.00 times parthenogenetic RWW, respectively ( < 0.01). The results show that had low expression in parthenogenetic RWW ovarian tissue, and was expressed normally. Our study suggests that the chromosomal unipolar division and deletion of may cause parthenogenesis in RWW.
稻水象甲分为三倍体孤雌生殖和二倍体两性生殖两种种群类型。在本研究中，我们通过免疫染色标记孤雌生殖稻水象甲卵母细胞的染色体和微管，探究了三倍体孤雌生殖稻水象甲（中国北京海淀区上庄镇）的减数分裂。免疫染色结果显示，三倍体孤雌生殖稻水象甲卵母细胞中形成了典型的减数分裂纺锤体，但染色体仅在一极分离，这意味着孤雌生殖稻水象甲在卵子发生过程中存在染色体单极分裂。此外，我们克隆了孤雌生殖稻水象甲 和 的保守序列，并基于孤雌生殖稻水象甲序列设计引物，通过定量PCR（Q-PCR）检测表达模式。Q-PCR结果表明，两性生殖稻水象甲卵巢组织中 和 的表达分别是孤雌生殖稻水象甲的0.98倍和10000.00倍（ < 0.01）。结果显示， 在孤雌生殖稻水象甲卵巢组织中表达较低，而 表达正常。我们的研究表明，染色体单极分裂和 的缺失可能导致稻水象甲的孤雌生殖。
