Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Osteoarthritis and Musculoskeleton Research Unit, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Molecules. 2021 Mar 24;26(7):1831. doi: 10.3390/molecules26071831.
Andrographolide is a labdane diterpenoid herb, which is isolated from the leaves of and widely used for its potential medical properties. However, there are no reports on the effects of andrographolide on the human suprapatellar fat pad of osteoarthritis patients. In the present study, our goal was to evaluate the innovative effects of andrographolide on viability and Tri-lineage differentiation of human mesenchymal stem cells from suprapatellar fat pad tissues. The results revealed that andrographolide had no cytotoxic effects when the concentration was less than 12.5 µM. Interestingly, andrographolide had significantly enhanced, dose dependent, osteogenesis and chondrogenesis as evidenced by a significantly intensified stain for Alizarin Red S, Toluidine Blue and Alcian Blue. Moreover, andrographolide can upregulate the expression of genes related to osteogenic and chondrogenic differentiation, including , , , and in mesenchymal stem cells from human suprapatellar fat pad tissues. In contrast, andrographolide suppressed adipogenic differentiation as evidenced by significantly diminished Oil Red O staining and expression levels for adipogenic-specific genes for and . These findings confirm that andrographolide can specifically enhance osteogenesis and chondrogenesis of mesenchymal stem cells from human suprapatellar fat pad tissues. It has potential as a therapeutic agent derived from natural sources for regenerative medicine.
穿心莲内酯是一种来源于穿心莲的 labdane 二萜类化合物，具有广泛的药用价值。然而，目前尚无关于穿心莲内酯对骨关节炎患者髌上脂肪垫组织中人类间充质干细胞活力和三系分化影响的报道。在本研究中，我们的目的是评估穿心莲内酯对人髌上脂肪垫间充质干细胞活力和三系分化的创新作用。结果表明，当浓度小于 12.5µM 时，穿心莲内酯对细胞没有细胞毒性作用。有趣的是，穿心莲内酯具有明显的增强作用，呈剂量依赖性，表现为茜素红 S、甲苯胺蓝和阿利新蓝染色明显增强。此外，穿心莲内酯可以上调与成骨和成软骨分化相关的基因的表达，包括 、 、 、 在人髌上脂肪垫间充质干细胞中。相比之下，穿心莲内酯抑制脂肪生成分化，表现为油红 O 染色明显减少，脂肪生成特异性基因 和 的表达水平降低。这些发现证实了穿心莲内酯可以特异性增强人髌上脂肪垫间充质干细胞的成骨和成软骨分化。它具有作为再生医学天然来源治疗剂的潜力。
