Chen Xiaoxue, Hu Yunan, Tian Simin, Han Beizhong
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Microorganisms. 2021 Mar 24;9(4):672. doi: 10.3390/microorganisms9040672.
In a raw-meat-processing environment, members of the Enterobacteriaceae family can coexist with to form dual-species biofilms, leading to a higher risk of food contamination. However, very little is known about the effect of inter-species interactions on dual-species biofilm formation. The aim of this study was to investigate the interactions between and raw-meat-processing environment isolates of in dual-species biofilms, by employing an untargeted metabolomics tool. Crystal violet staining assay showed that the biomass of the dual-species biofilm significantly increased and reached its maximum after incubation for 21 h, compared with that of single species grown alone. The number of in the dual-species biofilm was significantly higher than that of . Field emission scanning electron microscopy (FESEM) revealed that both species were evenly distributed, and were tightly wrapped by extracellular polymeric substances in the dual-species biofilms. Ultra-high-pressure liquid chromatography equipped with a quadrupole-time-of-flight mass spectrometer (UHPLC-Q-TOF MS) analysis exhibited a total of 8184 positive ions, and 6294 negative ions were obtained from all test samples. Multivariate data analysis further described altered metabolic profiling between mono- and dual-species biofilms. Further, 18 and 21 different metabolites in the dual-species biofilm were screened as biomarkers by comparing the mono-species biofilms of and , respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways that were exclusively upregulated in the dual-species biofilm included ABC transporters, amino acid metabolism, and the two-component signal transduction system. Our results contribute to a better understanding of the interactive behavior of inter-species biofilm communities, by discovering altered metabolic profiling.
在生肉加工环境中，肠杆菌科成员可与[具体菌种]共存以形成双物种生物膜，从而导致更高的食品污染风险。然而，关于种间相互作用对双物种生物膜形成的影响却知之甚少。本研究的目的是通过使用非靶向代谢组学工具，研究[具体菌种]与来自生肉加工环境的分离株在双物种生物膜中的相互作用。结晶紫染色试验表明，与单独生长的单物种相比，双物种生物膜的生物量显著增加，并在培养21小时后达到最大值。双物种生物膜中[具体菌种1]的数量显著高于[具体菌种2]。场发射扫描电子显微镜（FESEM）显示，两种菌均匀分布，并且在双物种生物膜中被细胞外聚合物紧密包裹。配备四极杆-飞行时间质谱仪（UHPLC-Q-TOF MS）的超高压液相色谱分析显示，所有测试样品共获得8184个正离子和6294个负离子。多变量数据分析进一步描述了单物种和双物种生物膜之间代谢谱的变化。此外，通过分别比较[具体菌种1]和[具体菌种2]的单物种生物膜，在双物种生物膜中筛选出18种和21种不同的代谢物作为生物标志物。在双物种生物膜中特异性上调的京都基因与基因组百科全书（KEGG）途径包括ABC转运蛋白、氨基酸代谢和双组分信号转导系统。我们的研究结果通过发现代谢谱的变化，有助于更好地理解种间生物膜群落的相互作用行为。
需注意，原文中部分菌种名称未给出具体内容，翻译时用[具体菌种]等表述替代。
