Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural Univ., Nanjing 210095, P.R. China.
Natl. Center of Meat Quality and Safety Control, Nanjing Agricultural Univ., Nanjing 210095, P.R. China.
J Food Sci. 2018 May;83(5):1326-1332. doi: 10.1111/1750-3841.14149. Epub 2018 Apr 18.
Bacterial biofilms formed on equipment surfaces are potential sources of cross-contamination and can be responsible for the spread of bacteria involved in food spoilage, such as some Enterobacteriaceae family members. In this study, the effect of chlorite-based disinfectants, including sodium hypochlorite (SH), chlorine dioxide (CD), strongly acidic electrolyzed water (StAEW), and neutral electrolyzed water (NEW), on inactivation of mono-biofilms of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter freundii was evaluated separately. All the strains were enumerated by the viable plate-count method after disinfection for 30 min. A comparison of the surviving cells after disinfection indicated that E. cloacae biofilms were more resistant to disinfectants than the biofilms of the other two strains, and treatment with all the disinfectants improved sanitizing. SH (200 mg/L) was the most effective in the reduction of cell number in the biofilms of all strains. Considering the safety of use and environmental protection, electrolyzed oxidizing water, especially StAEW, was a good suggestion for the inactivation of cells in K. oxytoca or C. freundii biofilms. These results suggest that the cells in biofilm of E. cloacae, K. oxytoca, and C. freundii were highly sensitive to chlorite-based disinfectants and provide insights into the efficacy of disinfectants in killing bacteria.
The Enterobacteriaceae biofilms formed on equipment surfaces, which can cause cross-contamination and food spoilage, are greatly challenging bacterial contaminants of food products. Electrolyzed oxidizing water is a novel, environmentally friendly disinfectant that can effectively treat Enterobacteriaceae biofilms. The results of this study may be used to design effective measures to disinfect biofilms on equipment contact surfaces.
在设备表面形成的细菌生物膜是潜在的交叉污染源，可能导致与食物变质有关的细菌（如某些肠杆菌科家族成员）的传播。在这项研究中，分别评估了基于亚氯酸盐的消毒剂（包括次氯酸钠 (SH)、二氧化氯 (CD)、强酸性电解水 (StAEW) 和中性电解水 (NEW)) 对阴沟肠杆菌、产酸克雷伯菌和弗氏柠檬酸杆菌单生物膜的灭活作用。所有菌株在消毒 30 分钟后均通过活菌平板计数法进行计数。消毒后存活细胞的比较表明，阴沟肠杆菌生物膜比其他两种生物膜对消毒剂更具抵抗力，所有消毒剂的处理均提高了消毒效果。SH（200mg/L）在减少所有菌株生物膜中细胞数量方面最有效。考虑到使用安全性和环境保护，电解氧化水，尤其是 StAEW，是灭活产酸克雷伯菌或弗氏柠檬酸杆菌生物膜中细胞的良好建议。这些结果表明，阴沟肠杆菌、产酸克雷伯菌和弗氏柠檬酸杆菌生物膜中的细胞对基于亚氯酸盐的消毒剂高度敏感，为消毒剂杀灭细菌的效果提供了深入了解。
在设备表面形成的肠杆菌科生物膜会导致交叉污染和食物变质，是食品产品中极具挑战性的细菌污染物。电解氧化水是一种新型环保消毒剂，可有效处理肠杆菌科生物膜。本研究的结果可用于设计有效的设备接触表面生物膜消毒措施。
