Yun Hyun Seok, Oh Jisun, Lim Ji Sun, Kim Hyo Jung, Kim Jong-Sang
School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea.
Institute of Agriculture Science and Technology, Kyungpook National University, Daegu 41566, Korea.
Insects. 2021 Mar 29;12(4):297. doi: 10.3390/insects12040297.
The aim of this study was to compare the anti-inflammatory effect of wasp venom (WV) from the yellow-legged hornet () with that of bee venom (BV) on BV-2 murine microglial cells. WV was collected from the venom sac, freeze-dried, and used for examinations. WV and BV were non-toxic to BV-2 cells at concentrations of 160 and 12 µg/mL or lower, respectively. Treatment with WV reduced the secretion of nitric oxide and proinflammatory cytokines, including interleukin-6 and tumor necrosis factor alpha, from BV-2 cells activated by lipopolysaccharide (LPS). Western blot analysis revealed that WV and BV decreased the expression levels of inflammation markers, including inducible nitric oxide synthase and cyclooxygenase-2. In addition, WV decreased the nuclear translocation of nuclear factor κB (NF-κB), which is a key transcription factor in the regulation of cellular inflammatory response. Cumulatively, the results demonstrated that WV inhibited LPS-induced neuroinflammation in microglial cells by suppressing the NF-κB-mediated signaling pathway, which warrants further studies to confirm its therapeutic potential for neurodegenerative diseases.
本研究旨在比较黄脚胡蜂黄蜂毒液（WV）与蜜蜂毒液（BV）对BV-2小鼠小胶质细胞的抗炎作用。WV从毒囊中收集，冻干后用于各项检测。WV和BV分别在浓度为160和12 µg/mL或更低时对BV-2细胞无毒。用WV处理可减少脂多糖（LPS）激活的BV-2细胞中一氧化氮和促炎细胞因子（包括白细胞介素-6和肿瘤坏死因子α）的分泌。蛋白质免疫印迹分析显示，WV和BV降低了炎症标志物（包括诱导型一氧化氮合酶和环氧化酶-2）的表达水平。此外，WV减少了核因子κB（NF-κB）的核转位，NF-κB是调节细胞炎症反应的关键转录因子。总体而言，结果表明WV通过抑制NF-κB介导的信号通路抑制LPS诱导的小胶质细胞神经炎症，这值得进一步研究以证实其对神经退行性疾病的治疗潜力。
