Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou 510663, China.
Genes (Basel). 2021 Mar 29;12(4):504. doi: 10.3390/genes12040504.
spores (GLS), the mature germ cells ejected from the abaxial side of the pileus, have diverse pharmacological effects. However, the genetic regulation of sporulation in this fungus remains unknown. Here, samples corresponding to the abaxial side of the pileus were collected from strain YW-1 at three sequential developmental stages and were then subjected to a transcriptome assay. We identified 1598 differentially expressed genes (DEGs) and found that the genes related to carbohydrate metabolism were strongly expressed during spore morphogenesis. In particular, genes involved in trehalose and malate synthesis were upregulated, implying the accumulation of specific carbohydrates in mature spores. Furthermore, the expression of genes involved in triterpenoid and ergosterol biosynthesis was high in the young fruiting body but gradually decreased with sporulation. Finally, spore development-related regulatory pathways were explored by analyzing the DNA binding motifs of 24 transcription factors that are considered to participate in the control of sporulation. Our results provide a dataset of dynamic gene expression during sporulation in . They also shed light on genes potentially involved in transcriptional regulation of the meiotic process, metabolism pathways in energy provision, and ganoderic acids and ergosterol biosynthesis.
担孢子（GLS）是从菌盖的背面排出的成熟生殖细胞，具有多种药理学作用。然而，该真菌的孢子形成的遗传调控仍然未知。在这里，从菌株 YW-1 的三个连续发育阶段收集了与菌盖背面相对应的样本，并进行了转录组分析。我们鉴定了 1598 个差异表达基因（DEGs），并发现与碳水化合物代谢相关的基因在孢子形态发生过程中强烈表达。特别是，参与海藻糖和苹果酸合成的基因上调，表明成熟孢子中积累了特定的碳水化合物。此外，在幼担子果中，参与三萜和麦角固醇生物合成的基因表达水平较高，但随着孢子形成逐渐降低。最后，通过分析被认为参与孢子形成调控的 24 个转录因子的 DNA 结合基序，探讨了与孢子发育相关的调控途径。我们的研究结果提供了担子果在孢子形成过程中动态基因表达的数据集。它们还揭示了可能参与减数分裂过程转录调控、能量供应代谢途径以及灵芝酸和麦角固醇生物合成的基因。
