School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
G3 (Bethesda). 2021 Dec 8;11(12). doi: 10.1093/g3journal/jkab337.
Ganoderma leucocontextum, a newly discovered species of Ganodermataceae in China, has diverse pharmacological activities. Ganoderma leucocontextum was widely cultivated in southwest China, but the systematic genetic study has been impeded by the lack of a reference genome. Herein, we present the first whole-genome assembly of G. leucocontextum based on the Illumina and Nanopore platform from high-quality DNA extracted from a monokaryon strain (DH-8). The generated genome was 50.05 Mb in size with an N50 scaffold size of 3.06 Mb, 78,206 coding sequences, and 13,390 putative genes. Genome completeness was assessed using the Benchmarking Universal Single-Copy Orthologs (BUSCO) tool, which identified 96.55% of the 280 Fungi BUSCO genes. Furthermore, differences in functional genes of secondary metabolites (terpenoids) were analyzed between G. leucocontextum and Ganoderma lucidum. Ganoderma leucocontextum has more genes related to terpenoids synthesis compared to G. lucidum, which may be one of the reasons why they exhibit different biological activities. This is the first genome assembly and annotation for G. leucocontextum, which would enrich the toolbox for biological and genetic studies in G. leucocontextum.
中国新发现的灵芝科真菌——糙皮灵芝具有多种药理活性。糙皮灵芝在中国西南地区广泛栽培，但由于缺乏参考基因组，系统的遗传研究受到阻碍。在此，我们基于从单核菌株（DH-8）提取的高质量 DNA，使用 Illumina 和 Nanopore 平台首次完成了糙皮灵芝的全基因组组装。生成的基因组大小为 50.05 Mb，N50 支架大小为 3.06 Mb，包含 78206 个编码序列和 13390 个推定基因。使用基准通用单拷贝同源物 (BUSCO) 工具评估基因组完整性，该工具鉴定了 280 个真菌 BUSCO 基因中的 96.55%。此外，还分析了糙皮灵芝和灵芝中次生代谢物（萜类化合物）功能基因的差异。与灵芝相比，糙皮灵芝中与萜类化合物合成相关的基因更多，这可能是它们表现出不同生物活性的原因之一。这是糙皮灵芝的第一个基因组组装和注释，将丰富糙皮灵芝的生物学和遗传学研究工具包。
