CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal.
Cellular Proteomics Research, Helmholtz Centre for Infection Research, Inhoffenstraße. 7, 38124 Braunschweig, Germany.
Int J Mol Sci. 2021 Mar 25;22(7):3370. doi: 10.3390/ijms22073370.
We focus on the stalked goose barnacle adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat for feeding and reproduction. The protein composition of the cement multicomplex and adhesive gland was quantitatively studied using shotgun proteomic analysis. Overall, 11,795 peptide sequences were identified in the gland and 2206 in the cement, clustered in 1689 and 217 proteinGroups, respectively. Cement specific adhesive proteins (CPs), proteases, protease inhibitors, cuticular and structural proteins, chemical cues, and many unannotated proteins were found, among others. In the cement, CPs were the most abundant (80.5%), being the bulk proteins CP100k and -52k the most expressed of all, and CP43k-like the most expressed interfacial protein. Unannotated proteins comprised 4.7% of the cement proteome, ranking several of them among the most highly expressed. Eight of these proteins showed similar physicochemical properties and amino acid composition to known CPs and classified through Principal Components Analysis (PCA) as new CPs. The importance of PCA on the identification of unannotated non-conserved adhesive proteins, whose selective pressure is on their relative amino acid abundance, was demonstrated.
我们专注于柄海鞘贻贝的粘附系统，这是一种对基质机会主义、选择性较低的物种，附着在海洋中各种漂浮物体上。粘附是贻贝的一种适应性特征，确保它们在栖息地中能够充分定位，以进行觅食和繁殖。我们使用鸟枪法蛋白质组学分析对水泥多复合物和附腺的蛋白质组成进行了定量研究。总的来说，在附腺中鉴定出了 11795 条肽序列，在水泥中鉴定出了 2206 条肽序列，分别聚类为 1689 和 217 个蛋白质组。发现了水泥特有的粘附蛋白（CPs）、蛋白酶、蛋白酶抑制剂、表皮和结构蛋白、化学线索和许多未注释的蛋白质等。在水泥中，CPs 最为丰富（80.5%），CP100k 和 -52k 是所有蛋白中表达量最高的，CP43k 样蛋白是表达量最高的界面蛋白。未注释的蛋白质占水泥蛋白质组的 4.7%，其中有几种蛋白质的表达量排名很高。这 8 种蛋白质具有与已知 CPs 相似的物理化学性质和氨基酸组成，并通过主成分分析（PCA）分类为新的 CPs。PCA 对识别未注释的非保守粘附蛋白非常重要，这些蛋白的选择压力在于它们相对氨基酸丰度。
