Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China.
Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Mar Drugs. 2020 Mar 31;18(4):186. doi: 10.3390/md18040186.
Barnacles represent one of the model organisms used for antifouling research, however, knowledge regarding the molecular mechanisms underlying barnacle cyprid cementation is relatively scarce. Here, RNA-seq was used to obtain the transcriptomes of the cement glands where adhesive is generated and the remaining carcasses of cyprids. Comparative transcriptomic analysis identified 9060 differentially expressed genes, with 4383 upregulated in the cement glands. Four cement proteins, named Mvcp113k, Mvcp130k, Mvcp52k and Mvlcp1-122k, were detected in the cement glands. The salivary secretion pathway was significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the differentially expressed genes, implying that the secretion of cyprid adhesive might be analogous to that of saliva. Lysyl oxidase had a higher expression level in the cement glands and was speculated to function in the curing of cyprid adhesive. Furthermore, the KEGG enrichment analysis of the 352 proteins identified in the cement gland proteome partially confirmed the comparative transcriptomic results. These results present insights into the molecular mechanisms underlying the synthesis, secretion and curing of barnacle cyprid adhesive and provide potential molecular targets for the development of environmentally friendly antifouling compounds.
藤壶是用于防污研究的模式生物之一，然而，有关藤壶幼体胶着的分子机制的知识相对较少。在这里，RNA-seq 被用于获得产生粘性物质的胶腺和剩余幼体残骸的转录组。比较转录组分析鉴定出 9060 个差异表达基因，其中 4383 个在胶腺中上调。在胶腺中检测到四种胶蛋白，分别命名为 Mvcp113k、Mvcp130k、Mvcp52k 和 Mvlcp1-122k。京都基因与基因组百科全书（KEGG）对差异表达基因的富集分析表明，唾液分泌途径显著富集，这表明藤壶胶的分泌可能类似于唾液的分泌。赖氨酰氧化酶在胶腺中的表达水平较高，推测其在胶腺胶的固化中起作用。此外，胶腺蛋白质组中鉴定的 352 种蛋白质的 KEGG 富集分析部分证实了比较转录组结果。这些结果为藤壶幼体胶的合成、分泌和固化的分子机制提供了新的认识，并为开发环保型防污化合物提供了潜在的分子靶标。
