Normand Anne-Cécile, Imbert Sébastien, Brun Sophie, Al-Hatmi Abdullah M S, Chryssanthou Erja, Cassaing Sophie, Schuttler Christine, Hasseine Lilia, Mahinc Caroline, Costa Damien, Bonnal Christine, Ranque Stéphane, Sautour Marc, Rubio Elisa, Delhaes Laurence, Riat Arnaud, Sendid Boualem, Kristensen Lise, Brandenberger Marcel, Guitard Juliette, Packeu Ann, Piarroux Renaud, Fekkar Arnaud
AP-HP, Groupe Hospitalier La Pitié-Salpêtrière, Service de Parasitologie Mycologie, 75013 Paris, France.
AP-HP, Hôpital Avicenne, Service de Parasitologie-Mycologie, 93000 Bobigny, France.
J Fungi (Basel). 2021 Mar 25;7(4):246. doi: 10.3390/jof7040246.
spp. are widespread environmental fungi as well as pathogens that can affect plants, animals and humans. Yet the epidemiology of human fusariosis is still cloudy due to the rapidly evolving taxonomy. The Mass Spectrometry Identification database (MSI) has been developed since 2017 in order to allow a fast, accurate and free-access identification of fungi by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Taking advantage of the MSI database user network, we aim to study the species distribution of spp. isolates in an international multicenter prospective study. This study also allowed the assessment of the abilities of miscellaneous techniques to identify isolates at the species level. The identification was performed by PCR-sequencing and phylogenic-tree approach. Both methods are used as gold standard for the evaluation of mass spectrometry. Identification at the species complex was satisfactory for all the tested methods. However, identification at the species level was more challenging and only 32% of the isolates were correctly identified with the National Center for Biotechnology Information (NCBI) DNA database, 20% with the Bruker MS database and 43% with the two MSI databases. Improvement of the mass spectrometry database is still needed to enable precise identification at the species level of any isolates encountered either in human pathology or in the environment.
镰孢菌属是广泛存在的环境真菌以及病原体，可影响植物、动物和人类。然而，由于分类学的快速发展，人类镰刀菌病的流行病学仍不明确。自2017年以来，质谱鉴定数据库（MSI）已被开发出来，以便通过基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱法对真菌进行快速、准确且免费的鉴定。利用MSI数据库用户网络，我们旨在通过一项国际多中心前瞻性研究来研究镰孢菌属分离株的物种分布。这项研究还允许评估多种技术在物种水平上鉴定镰孢菌分离株的能力。鉴定通过PCR测序和系统发育树方法进行。这两种方法都被用作评估质谱的金标准。对于所有测试方法，在物种复合体水平上的鉴定是令人满意的。然而，在物种水平上的鉴定更具挑战性，只有32%的分离株通过美国国立生物技术信息中心（NCBI）DNA数据库被正确鉴定，20%通过布鲁克质谱数据库被正确鉴定，43%通过两个MSI数据库被正确鉴定。仍需要改进质谱数据库，以便能够在物种水平上精确鉴定在人类病理学或环境中遇到的任何镰孢菌分离株。
