用于组织工程的PLGA支架结构与孔隙率的广泛多工具研究

Wide-Ranging Multitool Study of Structure and Porosity of PLGA Scaffolds for Tissue Engineering.

作者信息

Buzmakov Alexey V, Dunaev Andrey G, Krivonosov Yuriy S, Zolotov Denis A, Dyachkova Irina G, Krotova Larisa I, Volkov Vladimir V, Bodey Andrew J, Asadchikov Victor E, Popov Vladimir K

机构信息

Institute of Photon Technologies of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Pionerskaya 2, Troitsk, 108840 Moscow, Russia.

Diamond Light Source, Harwell Oxford Campus, Didcot OX11 0DE, UK.

出版信息

Polymers (Basel). 2021 Mar 25;13(7):1021. doi: 10.3390/polym13071021.

DOI:10.3390/polym13071021

PMID:33806130

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8037117/

Abstract

In this study, the nanoscale transformation of the polylactic-co-glycolic acid (PLGA) internal structure, before and after its supercritical carbon dioxide (sc-CO) swelling and plasticization, followed by foaming after a CO pressure drop, was studied by small-angle X-ray scattering (SAXS) for the first time. A comparative analysis of the internal structure data and porosity measurements for PLGA scaffolds, produced by sc-CO processing, on a scale ranging from 0.02 to 1000 μm, was performed by SAXS, helium pycnometry (HP), mercury intrusion porosimetry (MIP) and both "lab-source" and synchrotron X-ray microtomography (micro-CT). This approach opens up possibilities for the wide-scale evaluation, computer modeling, and prediction of the physical and mechanical properties of PLGA scaffolds, as well as their biodegradation behavior in the body. Hence, this study targets optimizing the process parameters of PLGA scaffold fabrication for specific biomedical applications.

摘要

在本研究中，首次通过小角X射线散射（SAXS）研究了聚乳酸-乙醇酸共聚物（PLGA）在超临界二氧化碳（sc-CO₂）溶胀和增塑前后的内部结构纳米级转变，随后在CO₂压力降后进行发泡。通过SAXS、氦比重瓶法（HP）、压汞法（MIP）以及“实验室源”和同步加速器X射线显微断层扫描（micro-CT），对通过sc-CO₂处理制备的PLGA支架在0.02至1000μm范围内的内部结构数据和孔隙率测量进行了对比分析。这种方法为广泛评估、计算机建模以及预测PLGA支架的物理和机械性能及其在体内的生物降解行为开辟了可能性。因此，本研究旨在针对特定生物医学应用优化PLGA支架制造的工艺参数。