Schardosim M, Soulié J, Poquillon D, Cazalbou S, Duployer B, Tenailleau C, Rey C, Hübler R, Combes C
CIRIMAT, Université de Toulouse, CNRS, INPT, UPS, ENSIACET, 4 allée Emile Monso, CS 44362, 31030 Toulouse cedex 4, France; PUCRS, GEPSI-LMN, Porto Alegre, Brazil.
CIRIMAT, Université de Toulouse, CNRS, INPT, UPS, ENSIACET, 4 allée Emile Monso, CS 44362, 31030 Toulouse cedex 4, France.
Mater Sci Eng C Mater Biol Appl. 2017 Aug 1;77:731-738. doi: 10.1016/j.msec.2017.03.302. Epub 2017 Apr 2.
This paper focuses on the fabrication of three-dimensional porous PLGA-biomimetic carbonated apatite composite scaffolds by freeze-casting and using dimethyl carbonate as a solvent. Several charge/polymer ratios were tested in order to finely understand the influence of the filler rate on the scaffold porosity and mechanical and degradation properties using complementary characterization techniques (SEM, mercury porosimetry and X-ray microtomography). It was demonstrated that the apatite ratio within the composite scaffold has a strong influence in terms of architecture, material cohesion, mechanical properties and in vitro degradation properties. An optimum biomimetic apatite ratio was reached to combine good mechanical properties (higher rigidity) and material cohesion. In vitro degradation studies showed that higher apatite filler rates limited PLGA degradation and enhanced the hydrophilicity of the scaffolds which is expected to improve the biological properties of the scaffolds in addition to the bioactivity related to the presence of the apatite analogous to bone mineral.
本文重点研究了通过冷冻浇铸法并使用碳酸二甲酯作为溶剂来制备三维多孔聚乳酸-羟基乙酸共聚物-仿生碳酸化磷灰石复合支架。测试了几种电荷/聚合物比例，以便使用互补表征技术（扫描电子显微镜、压汞法和X射线显微断层扫描）来深入了解填料比例对支架孔隙率、力学性能和降解性能的影响。结果表明，复合支架中的磷灰石比例在结构、材料内聚力、力学性能和体外降解性能方面具有强烈影响。达到了最佳的仿生磷灰石比例，以兼具良好的力学性能（更高的刚性）和材料内聚力。体外降解研究表明，较高的磷灰石填料比例限制了聚乳酸-羟基乙酸共聚物的降解，并增强了支架的亲水性，除了与类似骨矿物质的磷灰石存在相关的生物活性外，这有望改善支架的生物学性能。
