Ogurcov Sergei, Shulman Iliya, Garanina Ekaterina, Sabirov Davran, Baichurina Irina, Kuznetcov Maxim, Masgutova Galina, Kostennikov Alexander, Rizvanov Albert, James Victoria, Mukhamedshina Yana
Neurosurgical Department No. 2, Republic Clinical Hospital, 420138 Kazan, Russia.
Clinical Research Center for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.
Brain Sci. 2021 Mar 4;11(3):322. doi: 10.3390/brainsci11030322.
. Despite considerable interest in the search for a spinal cord injury (SCI) therapy, there is a critical need to develop a panel of diagnostic biomarkers to determine injury severity. In this regard, there is a requirement for continuing research into the fundamental processes of neuroinflammatory and autoimmune reactions in SCI, identifying changes in the expression of cytokines. . In this pilot study, an extended multiplex analysis of the cytokine profiles in the serum of patients at 2 weeks post-SCI ( = 28) was carried out, together with an additional assessment of neuron-specific enolase (NSE) and vascular endothelial growth factor (VEGF) levels by enzyme-linked immunosorbent assay. A total of 16 uninjured subjects were enrolled as controls. . The data obtained showed a large elevation of IFNγ (>52 fold), CCL27 (>13 fold), and CCL26 (>8 fold) 2 weeks after SCI. The levels of cytokines CXCL5, CCL11, CXCL11, IL10, TNFα, and MIF were different between patients with baseline American Spinal Injury Association Impairment Scale (AIS) grades of A or B, whilst IL2 (>2 fold) and MIP-3a (>6 fold) were significantly expressed in the cervical and thoracic regions. There was a trend towards increasing levels of NSE. However, the difference in NSE was lost when the patient set was segregated based on AIS group. . Our pilot research demonstrates that serum concentrations of cytokines can be used as an affordable and rapid detection tool to accurately stratify SCI severity in patients.
尽管人们对寻找脊髓损伤（SCI）治疗方法有着浓厚兴趣，但迫切需要开发一组诊断生物标志物来确定损伤严重程度。在这方面，需要持续研究SCI中神经炎症和自身免疫反应的基本过程，确定细胞因子表达的变化。在这项初步研究中，对SCI后2周患者（n = 28）血清中的细胞因子谱进行了扩展多重分析，并通过酶联免疫吸附测定法对神经元特异性烯醇化酶（NSE）和血管内皮生长因子（VEGF）水平进行了额外评估。共招募了16名未受伤的受试者作为对照。获得的数据显示，SCI后2周时，IFNγ（>52倍）、CCL27（>13倍）和CCL26（>8倍）大幅升高。基线美国脊髓损伤协会损伤量表（AIS）分级为A或B的患者中，细胞因子CXCL5、CCL11、CXCL11、IL10、TNFα和MIF的水平有所不同，而IL2（>2倍）和MIP-3a（>6倍）在颈部和胸部区域显著表达。NSE水平有升高趋势。然而，根据AIS组对患者进行分类时，NSE的差异消失了。我们的初步研究表明，细胞因子的血清浓度可作为一种经济实惠且快速的检测工具，用于准确分层患者的SCI严重程度。
