Sabirov Davran, Ogurcov Sergei, Shulman Ilya, Kabdesh Ilyas, Garanina Ekaterina, Sufianov Albert, Rizvanov Albert, Mukhamedshina Yana
OpenLab "Gene and Cell Technologies", Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420008 Kazan, Russia.
Neurosurgical Department No. 2, Republic Clinical Hospital, 420138 Kazan, Russia.
Biomedicines. 2023 Sep 26;11(10):2641. doi: 10.3390/biomedicines11102641.
Cytokines are actively involved in the regulation of the inflammatory and immune responses and have crucial importance in the outcome of spinal cord injuries (SCIs). Examining more objective and representative indicators of the patient's condition is still required to reveal the fundamental patterns of the abovementioned posttraumatic processes, including the identification of changes in the expression of cytokines.
We performed a dynamic (3, 7, and 14 days post-injury (dpi)) extended multiplex analysis of cytokine profiles in both CSF and blood serum of SCI patients with baseline American Spinal Injury Association Impairment Scale grades of A.
The data obtained showed a large elevation of IL6 (>58 fold) in CSF and IFN-γ (>14 fold) in blood serum at 3 dpi with a downward trend as the post-traumatic period increases. The level of cytokine CCL26 was significantly elevated in both CSF and blood serum at 3 days post-SCI, while other cytokines did not show the same trend in the different biosamples.
The dynamic changes in cytokine levels observed in our study can explore the relationships with the SCI region and injury severity, paving the way for a better understanding of the pathophysiology of SCI and potentially more targeted and personalized therapeutic interventions.
细胞因子积极参与炎症和免疫反应的调节，在脊髓损伤（SCI）的预后中具有至关重要的意义。仍需要研究更客观、更具代表性的患者病情指标，以揭示上述创伤后过程的基本模式，包括确定细胞因子表达的变化。
我们对美国脊髓损伤协会损伤分级为A的SCI患者的脑脊液和血清中的细胞因子谱进行了动态（伤后3、7和14天）扩展多重分析。
获得的数据显示，伤后3天时脑脊液中IL6大幅升高（>58倍），血清中IFN-γ大幅升高（>14倍），且随着创伤后时间的增加呈下降趋势。SCI后3天时，脑脊液和血清中细胞因子CCL26的水平均显著升高，而其他细胞因子在不同生物样本中未显示出相同趋势。
我们研究中观察到的细胞因子水平的动态变化可以探索与SCI区域和损伤严重程度的关系，为更好地理解SCI的病理生理学以及潜在的更具针对性和个性化的治疗干预铺平道路。
