Noll Matthias, Jäger Michael, Lux Leonie, Buettner Christian, Axt-Gadermann Michaela
Institute for Bioanalysis, Department of Applied Sciences, Coburg University of Applied Sciences and Arts, 96450 Coburg, Germany.
Microorganisms. 2021 Mar 4;9(3):527. doi: 10.3390/microorganisms9030527.
Atopic dermatitis (AD) is a widespread chronic inflammatory dermatologic disorder. This randomized, double-blind study aims to evaluate the effect of synbiotic baths with a defined mixture of six viable lactic acid bacteria (LAB) and prebiotics, without bacteria and prebiotics and placebo baths without prebiotics and bacteria to treat AD patients over a period of 14 days. Therefore, AD patients were randomly assigned into three groups using synbiotic ( = 7), prebiotics ( = 8) or placebo baths ( = 7). Severity of AD was evaluated over time by using severity scoring of atopic dermatitis (SCORAD) and by patient questionnaires. In addition, microbiome on eczematous skin surface was sampled by swaps from each patient before the bath treatment, and after 9, 11 and 14 days of bath treatment. Thereafter, nucleic acids were extracted and the bacterial 16S rRNA gene was amplified via PCR for subsequent amplicon sequencing. Results showed a significantly reduced SCORAD over time of AD patients after daily synbiotic or prebiotic baths. Moreover, AD patients after daily synbiotic baths had a significantly improved pruritus and skin dryness and their bacterial microbiome was enriched by LAB. Taken together, a synbiotic bath is a promising topical skin application to alleviate AD.
特应性皮炎（AD）是一种广泛存在的慢性炎症性皮肤病。这项随机双盲研究旨在评估含有六种活性乳酸菌（LAB）和益生元的特定混合物的合生元浴、不含细菌和益生元的浴以及不含益生元和细菌的安慰剂浴在14天内治疗AD患者的效果。因此，AD患者被随机分为三组，分别使用合生元浴（n = 7）、益生元浴（n = 8）或安慰剂浴（n = 7）。通过使用特应性皮炎严重程度评分（SCORAD）和患者问卷随时间评估AD的严重程度。此外，在浴疗前以及浴疗9、11和14天后，通过对每位患者的湿疹皮肤表面进行擦拭取样来获取微生物群。此后，提取核酸，并通过PCR扩增细菌16S rRNA基因以进行后续的扩增子测序。结果显示，每日进行合生元浴或益生元浴后，AD患者的SCORAD随时间显著降低。此外，每日进行合生元浴的AD患者瘙痒和皮肤干燥症状明显改善，其细菌微生物群富含LAB。综上所述，合生元浴是一种有前景的局部皮肤应用方法，可缓解AD。
