Department of Anatomy, College of Korean Medicine, Kyung Hee University, 26, Kyunghee dae-ro, Dongdaemun-gu, Seoul 02447, Korea.
Department of Pharmaceutical Science, Jungwon University, 85, Munmu-ro, Goesan-eup, Goesan-gun, Chungbuk 28024, Korea.
Nutrients. 2021 Mar 4;13(3):839. doi: 10.3390/nu13030839.
(CP), or Korean red pinecone, is a cluster of fruit. CP has also been verified in several studies to have anti-oxidation, anti-fungal, anti-bacterial, and anti-melanogenic effects. However, anti-inflammatory effects have not yet been confirmed in the inflammatory responses of pinecones to allergic contact dermatitis. The purpose of this study is to prove the anti-inflammatory effect of CP on allergic contact dermatitis (ACD) in vitro and in vivo. CP inhibited the expression of TSLP, TARC, MCP-1, TNF-α, and IL-6 in TNF-α/IFN-γ-stimulated HaCaT cells and MCP-1, GM-CSF, TNF-α, IL-6, and IL-8 in PMACI (phorbol-12-myristate-13-acetate plus A23187)-stimulated HMC-1 cells. CP inhibited the phosphorylation of mitogen-activated protein kinase (MAPKs), as well as the translocation of NF-κB on TNF-α/IFN-γ stimulated in HaCaT cells. In vivo, CP decreased major symptoms of ACD, levels of IL-6 in skin lesion, thickening of the epidermis and dermis, infiltration of eosinophils and mast cells, and the infiltration of CD4 T cells and CD8 T cells. This result suggests that CP represents a potential alternative medicine to ACD for diseases such as chronic skin inflammation.
CP，又称朝鲜红松果，是一种果实簇。已有多项研究证实 CP 具有抗氧化、抗真菌、抗细菌和抗黑色素生成作用。然而，在松果体对过敏性接触性皮炎的炎症反应中，其抗炎作用尚未得到证实。本研究旨在证明 CP 对体外和体内过敏性接触性皮炎（ACD）的抗炎作用。CP 抑制了 TNF-α/IFN-γ 刺激的 HaCaT 细胞中 TSLP、TARC、MCP-1、TNF-α 和 IL-6 的表达，以及 PMACI（佛波醇-12-肉豆蔻酸-13-醋酸酯加 A23187）刺激的 HMC-1 细胞中 MCP-1、GM-CSF、TNF-α、IL-6 和 IL-8 的表达。CP 抑制了 MAPK 的磷酸化，以及 TNF-α/IFN-γ 刺激的 HaCaT 细胞中 NF-κB 的转位。在体内，CP 减少了 ACD 的主要症状，皮肤损伤中 IL-6 的水平，表皮和真皮的增厚，嗜酸性粒细胞和肥大细胞的浸润，以及 CD4 T 细胞和 CD8 T 细胞的浸润。这一结果表明，CP 为治疗慢性皮肤炎症等疾病提供了一种有潜力的替代药物。
