College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China.
Chem Biol Interact. 2019 Apr 1;302:156-163. doi: 10.1016/j.cbi.2019.01.037. Epub 2019 Feb 2.
Penta-O-galloyl-β-d-glucose (PGG) was purified and identified from Radix Paeoniae Alba by HSCCC and HPLC/ESI-MS, and its inhibitory effects on adipogenesis and TNF-α-induced inflammation were assessed in 3T3-L1 cell line. The results showed that PGG dose-dependently reduced intracellular lipids accumulation, and this involved decrease the expression levels of major adipogenic markers, PPARγ, C/EBP α, through MAPKs inhibition. This was accompanied by a reduction of lipogenic genes, ACC, FAS, and SCD-1, involved in fatty acid synthesis. Furthermore, PGG also inhibited TNF-α-induced expression of inflammatory cytokines including IL-6 and MCP-1 in the matured 3T3-L1 adipocytes. The inhibitions were likely mediated by blocking the MAPKs and NF-κB activation. These findings highlighted that PGG could serve as a potent therapeutic agent for controlling obesity and obesity-related chronic inflammation.
五没食子酰葡萄糖（PGG）通过 HSCCC 和 HPLC/ESI-MS 从白芍中分离纯化得到，并在 3T3-L1 细胞系中评估了其对脂肪生成和 TNF-α 诱导的炎症的抑制作用。结果表明，PGG 呈剂量依赖性降低细胞内脂质积累，这涉及通过 MAPKs 抑制降低主要脂肪生成标志物 PPARγ、C/EBPα 的表达水平。这伴随着参与脂肪酸合成的脂肪生成基因 ACC、FAS 和 SCD-1 的减少。此外，PGG 还抑制了 TNF-α 诱导的成熟 3T3-L1 脂肪细胞中炎症细胞因子（包括 IL-6 和 MCP-1）的表达。这种抑制可能是通过阻断 MAPKs 和 NF-κB 激活来介导的。这些发现强调了 PGG 可以作为控制肥胖和肥胖相关慢性炎症的有效治疗剂。
