Center for Medical Research, Medical University of Graz, 8010 Graz, Austria.
Department of Paediatric and Adolescent Surgery, Medical University of Graz, 8010 Graz, Austria.
Nutrients. 2021 Mar 23;13(3):1030. doi: 10.3390/nu13031030.
Environmental factors, including nutritional habits or birth mode, are known key determinants for intestinal microbial composition. Investigations of the intestinal microbiome in different species in a multiplicity of studies during recent decades have revealed differential microbial patterns and quantities along the gastrointestinal (GI) tract. Characterization of the microbial pattern in various aspects is a prerequisite for nutritional interventions. In this 16S rRNA amplicon-based approach, we present a characterization of the mucosa-associated microbiome in comparison with the luminal community of four infants at the time of the closure of ileostomies and perform a systematic characterization of the corresponding luminal and mucosal microbiome from jejunal, ileal and colonic regions, as well as collected feces in mice. The most dominant taxa in infant-derived samples altered due to individual differences, and in the mucosa, , , , and were the most abundant. Two less abundant taxa differed significantly between the mucosa and lumen. In murine samples, relative abundances differed significantly, mainly between the intestinal regions. Significant differences between mouse mucosa- and lumen-derived samples could be found in the observed species with a trend to lower estimated diversity in mucosa-derived samples, as well as in the relative abundance of individual taxa. In this study, we examined the difference between the mucosal and luminal bacterial colonization of the gastrointestinal tract in a small sample cohort of preterm infants. Individual differences were characterized and statistical significance was reached in two taxa (, ). The corresponding study on the different murine intestinal regions along the GI tract showed differences all over the intestinal region.
环境因素，包括营养习惯或分娩方式，是已知的肠道微生物组成的关键决定因素。在最近几十年的多项研究中，对不同物种的肠道微生物组进行了调查，揭示了沿胃肠道（GI）的不同微生物模式和数量。在各个方面对微生物模式进行特征描述是营养干预的前提。在这种基于 16S rRNA 扩增子的方法中，我们描述了四个闭瘘婴儿的回肠黏膜相关微生物组与腔微生物组的特征，并对相应的空肠、回肠和结肠区域以及收集的粪便中的黏膜和腔微生物组进行了系统的描述。在婴儿样本中，由于个体差异，最主要的分类群发生了改变，在黏膜中， 、 、 、 和 是最丰富的。两个较少的分类群在黏膜和腔之间有显著差异。在鼠类样本中，丰度差异显著，主要存在于肠道区域之间。在观察到的物种中，在黏膜和腔衍生样本之间存在显著差异，黏膜衍生样本的多样性估计值呈下降趋势，以及个别分类群的相对丰度也呈下降趋势。在这项研究中，我们检查了早产儿小样本队列的胃肠道黏膜和腔细菌定植的差异。在两个分类群（ 、 ）中，个体差异得到了描述，并且达到了统计学意义。对不同的鼠类肠道区域沿胃肠道的相应研究显示，整个肠道区域都存在差异。
