Pannuti A, Lanfrancone L, Pascucci A, Pelicci P G, La Mantia G, Lania L
Dipartimento di Genetica, Biologia Generale e Molecolare, University of Naples, Italy.
Nucleic Acids Res. 1988 May 25;16(10):4227-37. doi: 10.1093/nar/16.10.4227.
The finger motif is a tandemly repeated DNA-binding domain recently identified in the primary structure of several eukaryotic transcriptional regulatory proteins. It was first described for Xenopus TFIIIA, a factor required for transcription of 5S ribosomal genes and subsequently identified in regulatory proteins from other eukaryotic organisms including yeast, Drosophila and mammals. In this paper we report the isolation and characterization of two human cDNA clones both encoding for multifingered protein products. Transcriptional studies indicate that the two finger genes are expressed in a variety of human tissues. Moreover, upon in vitro induced terminal differentiation of human HL-60 and THP-1 myeloid cell lines the expression of both genes is drastically reduced. These data provide support for the existence of a human multigene family coding for regulatory finger proteins which are likely involved in the processes of cell differentiation and/or proliferation.
手指基序是最近在几种真核转录调节蛋白的一级结构中发现的串联重复DNA结合结构域。它最初是针对非洲爪蟾TFIIIA描述的，TFIIIA是5S核糖体基因转录所需的一种因子，随后在包括酵母、果蝇和哺乳动物在内的其他真核生物的调节蛋白中被鉴定出来。在本文中，我们报告了两个编码多手指蛋白产物的人类cDNA克隆的分离和特性。转录研究表明，这两个手指基因在多种人类组织中表达。此外，在体外诱导人类HL-60和THP-1髓系细胞系终末分化后，这两个基因的表达均大幅降低。这些数据为编码调节性手指蛋白的人类多基因家族的存在提供了支持，这些蛋白可能参与细胞分化和/或增殖过程。
