Gil Diogo, Cardoso Joana M S, Abrantes Isabel, Esteves Ivânia
Centre of Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
Plants (Basel). 2021 Mar 23;10(3):603. doi: 10.3390/plants10030603.
The root lesion is an economically important pest affecting a wide range of plants. The morphometry of five isolates, parasitizing potato roots in Portugal, was compared and variability within and between isolates was observed. Of the 15 characters assessed, vulva position (V%) in females and the stylet length in both females/males showed the lowest coefficient of intra and inter-isolate variability. Moreover, DNA sequencing of the internal transcribed spacers (ITS) genomic region and cytochrome c oxidase subunit 1 (COI) gene was performed, in order to evaluate the intraspecific genetic variability of this species. ITS revealed higher isolate genetic diversity than the COI gene, with 15 and 7 different haplotypes from the 15 ITS and 14 COI sequences, respectively. Intra- and inter-isolate genetic diversity was found considering both genomic regions. The differentiation of these isolates was not related with their geographical origin. In spite of the high intraspecific variability, phylogenetic analyses revealed that both ITS region and COI gene separate from other related species. Our findings contribute to increasing the understanding of variability.
根腐病菌是一种对经济有重要影响的害虫，可侵害多种植物。对葡萄牙寄生在马铃薯根部的五个分离株的形态测量进行了比较，并观察了分离株内部和之间的变异性。在评估的15个特征中，雌性外阴位置（V%）以及雌性/雄性的口针长度在分离株内部和之间显示出最低的变异系数。此外，对内部转录间隔区（ITS）基因组区域和细胞色素c氧化酶亚基1（COI）基因进行了DNA测序，以评估该物种的种内遗传变异性。ITS显示出比COI基因更高的分离株遗传多样性，分别从15个ITS序列和14个COI序列中发现了15种和7种不同的单倍型。考虑到这两个基因组区域，发现了分离株内部和之间的遗传多样性。这些分离株的分化与其地理起源无关。尽管种内变异性很高，但系统发育分析表明，ITS区域和COI基因均与其他相关物种不同。我们的研究结果有助于增进对变异性的理解。
