Cohen L F, Lundgren D W, Farrell P M
Blood. 1976 Sep;48(3):469-75.
Previous studies have shown an abnormality of the spermidine-to-spermine (Spd/Spm) ratio in whole blood of cystic fibrosis homo-and heterozygotes. To investigate Spd and Spm distribution amoung blood components as a possible cause of the abnormality, blood was fractionated using Rabinowitz's glass bead technique and Boyum's Ficoll-Hypaque method. Free (unconjugated) polyamines were extracted with perchloric acid and quantitated on an amino acid analyzer. In controls, mean +/- SEM concentrations in nmoles/10(9) cells of Spd and Spm, respectively, were 1.02 +/- 0.08 and 0.894 +/- 0.28 for erythrocytes; 126 +/- 31 and 357 +/- 105 for lymphocytes; 36 +/- 16 and 240 +/- 33 for granulocytes; and less than 0.5 and less than 0.5 nmoles/ml for plasma. When converted to the concentration in whole blood, it was found that greater than 90% of Spd and over 70% of Spm was associated with erythrocytes. While the higher cellular concentration in leukocytes was not unexpected, the fact that Spd and Spm in whole blood were primarily associated with erythrocytes was a new finding. Comparison with controls revealed that the Spd/Spm ratio in both whole blood and erythrocytes was significantly higher in the group of cystic fibrosis patients.
先前的研究表明，囊性纤维化纯合子和杂合子的全血中精胺与精脒（Spd/Spm）的比例存在异常。为了研究Spd和Spm在血液成分中的分布情况，作为该异常可能的原因，采用Rabinowitz的玻璃珠技术和Boyum的Ficoll-泛影葡胺方法对血液进行了分离。用高氯酸提取游离（未结合）多胺，并在氨基酸分析仪上进行定量。在对照组中，红细胞中Spd和Spm的平均浓度（以nmol/10⁹细胞计，±SEM）分别为1.02±0.08和0.894±0.28；淋巴细胞中分别为126±31和357±105；粒细胞中分别为36±16和240±33；血浆中均小于0.5 nmol/ml。当换算为全血中的浓度时，发现超过90%的Spd和超过70%的Spm与红细胞相关。虽然白细胞中较高的细胞浓度在意料之中，但全血中的Spd和Spm主要与红细胞相关这一事实却是一个新发现。与对照组相比，囊性纤维化患者组的全血和红细胞中的Spd/Spm比值均显著更高。
