在四烷基铵盐溶液中，寡核苷酸双链体的热稳定性与序列无关：应用于鉴定重组DNA克隆。
The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.
作者信息
Jacobs K A, Rudersdorf R, Neill S D, Dougherty J P, Brown E L, Fritsch E F
机构信息
Genetics Institute Inc., Cambridge, MA 02140.
出版信息
Nucleic Acids Res. 1988 May 25;16(10):4637-50. doi: 10.1093/nar/16.10.4637.
Abstract
In solutions of tetraalkylammonium salts the melting temperature of oligonucleotide duplexes is independent of nucleotide sequence and thus GC content. Data quantitating the destabilizing effects of various mismatches in these solvents are also presented. The results are in accord with theories on DNA melting and establish conditions under which oligonucleotides can be used as hybridization probes with predictable and controllable specificity.
摘要
在四烷基铵盐溶液中，寡核苷酸双链体的解链温度与核苷酸序列无关，因此也与GC含量无关。本文还给出了定量这些溶剂中各种错配的去稳定化效应的数据。结果与DNA解链理论一致，并确定了寡核苷酸可作为具有可预测和可控特异性的杂交探针的条件。
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