在2'-位含有疏水性荧光染料的寡核苷酸衍生物的合成与性质
Synthesis and properties of oligonucleotide derivatives containing a hydrophobic fluorescent dye at the 2'-position.
作者信息
Mitsui T, Hayashi H, Yamana K, Nakano H
机构信息
Department of Applied Chemistry, Himeji Institute of Technology, Japan.
出版信息
Nucleic Acids Symp Ser. 1997(37):69-70.
PMID:9586003
Abstract
The oligonucleotides containing 2'-(6-dimethylamino-2-naphthamide)uridine [U(DAN)] at the different position were synthesized by the fully automated solid phase phosphoramidite methods. The UV melting profiles for the U(DAN)-modified oligonucleotides with complementary DNA exhibited sigmoidal curves whose shapes were similar to those for the unmodified duplexes.
摘要
通过全自动固相亚磷酰胺法合成了在不同位置含有2'-(6-二甲基氨基-2-萘甲酰胺)尿苷[U(DAN)]的寡核苷酸。U(DAN)修饰的寡核苷酸与互补DNA的紫外熔解曲线呈S形，其形状与未修饰的双链体相似。
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