GG、TG和CA对（CG）n序列的打断并不一定会阻止溶液中B型向Z型的转变。
Interruptions of (CG)n sequences by GG, TG and CA need not prevent B to Z transition in solution.
作者信息
Mishra R K, Latha P K, Brahmachari S K
机构信息
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
出版信息
Nucleic Acids Res. 1988 May 25;16(10):4651-65. doi: 10.1093/nar/16.10.4651.
Abstract
Oligonucleotides containing alternating purines-pyrimidines with AT base pairs have been shown to exist in the Z-form preferably in solid state. We report that oligodeoxyribonucleotides with GG, TG and CA interruptions in their alternating CG sequences can undergo B to Z transition in solution in the absence of any chemical modification or topological constraint. The sequences, d(CGCGCGGCGCGC) and d(CGTGCGCACG) have been synthesised and shown to adopt Z- conformation in presence of millimolar concentrations of Ni2+ under low water activity conditions. Significance of GG, TG and CA interruptions in the B to Z transition is discussed.
摘要
含有交替嘌呤-嘧啶且具有AT碱基对的寡核苷酸已被证明在固态时更倾向于以Z型存在。我们报道，在其交替的CG序列中含有GG、TG和CA间断的寡脱氧核糖核苷酸，在没有任何化学修饰或拓扑限制的情况下，能在溶液中发生从B型到Z型的转变。已合成了序列d(CGCGCGGCGCGC)和d(CGTGCGCACG)，并表明在低水活度条件下，在毫摩尔浓度的Ni2+存在时会采用Z构象。讨论了GG、TG和CA间断在从B型到Z型转变中的意义。
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