Z型聚（dG-dC）·聚（dG-dC）与二价金属离子的相互作用：通过红外光谱法确定结合位点的位置
Interaction of Z form poly(dG-dC).poly(dG-dC) with divalent metal ions: localization of the binding sites by I.R. spectroscopy.
作者信息
Taboury J A, Bourtayre P, Liquier J, Taillandier E
出版信息
Nucleic Acids Res. 1984 May 25;12(10):4247-58. doi: 10.1093/nar/12.10.4247.
Abstract
The secondary structures of poly(dG-dC).poly(dG-dC) in the presence of alcaline , alcaline earth and first row transition metal ions (Na+, Mg2+, Co2+, Ni2+) are investigated by infrared spectroscopy. The conformational transitions are studied as a function of the hydration of the polynucleotide and counter-ion nature and content. The use of selectively deuterated poly(dG-dC).poly(dG-dC) on the 8-carbon of guanines allows to show that a direct interaction occurs between the N7 site of guanines and the transition metal ions Co2+ and Ni2+. In the case of Mg2+, for high ion/nucleotide ratios, the interaction occurs essentially at the level of the phosphate groups. This interaction leads to a modification of the left-handed conformation. Based on the IR spectroscopy results, an explanation is proposed for the different efficiencies of these various ions to induce the B----Z transition.
摘要
通过红外光谱研究了聚（dG-dC）·聚（dG-dC）在碱性、碱土金属和第一过渡系金属离子（Na⁺、Mg²⁺、Co²⁺、Ni²⁺）存在下的二级结构。研究了构象转变与多核苷酸水合作用以及抗衡离子性质和含量的关系。使用在鸟嘌呤8位碳上选择性氘代的聚（dG-dC）·聚（dG-dC），可以表明鸟嘌呤的N7位点与过渡金属离子Co²⁺和Ni²⁺之间发生了直接相互作用。在Mg²⁺的情况下，对于高离子/核苷酸比率，相互作用主要发生在磷酸基团水平。这种相互作用导致左手构象的改变。基于红外光谱结果，对这些不同离子诱导B→Z转变的不同效率提出了一种解释。
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