Liu Tao, Ren Xiaoli, Cao Guangyun, Zhou Xia, Jin Linhong
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.
Microorganisms. 2021 Mar 31;9(4):724. doi: 10.3390/microorganisms9040724.
Bacterial canker disease caused by pv. (Psa) is a devastating disease of kiwifruit, which is severely limiting the development of the kiwifruit industry. Ethylicin is a broad-spectrum plant biomimetic fungicide. However, its application in the control of kiwifruit bacterial canker is rarely reported, and the mechanism of ethylicin on Psa remains unknown. In this study, we investigated the effect of ethylicin on Psa in vitro and in vivo and found that ethylicin can inhibit the growth of Psa and prevent the cankering in the plant stem. Mechanism investigation indicated that ethylicin acted by limiting the movement of Psa, destroying the cell membrane of Psa, and inhibiting the formation of Psa biofilm. In addition, it was also found through transcriptomics research that ethylicin can up-regulate the expression of genes related to protein export and biofilm formation- and down-regulate the expression of genes related to flagellar assembly in Psa. This study concluded that ethylicin can effectively inhibit Psa growth, and it could help to gain a better understanding of the mechanisms of ethylicin inhibiting Psa and provide practical data for the application of ethylicin as a highly potent agent for controlling the bacterial canker disease of kiwifruit.
由丁香假单胞菌猕猴桃致病变种（Psa）引起的细菌性溃疡病是猕猴桃的一种毁灭性病害，严重限制了猕猴桃产业的发展。乙蒜素是一种广谱植物源仿生杀菌剂。然而，其在防治猕猴桃细菌性溃疡病方面的应用鲜有报道，且乙蒜素对Psa的作用机制尚不清楚。在本研究中，我们研究了乙蒜素在体外和体内对Psa的影响，发现乙蒜素能够抑制Psa的生长并防止植物茎干溃疡。机制研究表明，乙蒜素通过限制Psa的移动、破坏Psa的细胞膜以及抑制Psa生物膜的形成发挥作用。此外，通过转录组学研究还发现，乙蒜素能够上调Psa中与蛋白质输出和生物膜形成相关基因的表达，并下调与鞭毛组装相关基因的表达。本研究得出结论，乙蒜素能够有效抑制Psa的生长，有助于更好地理解乙蒜素抑制Psa的机制，并为乙蒜素作为防治猕猴桃细菌性溃疡病的高效药剂的应用提供实际数据。
