Research Center for Engineering Technology of Kiwifruit, Institute of Crop Protection, College of Agriculture, Guizhou University, Guiyang 550025, China.
Teaching Experimental Field of Guizhou University, Guizhou University, Guiyang 550025, China.
Int J Mol Sci. 2021 Dec 24;23(1):207. doi: 10.3390/ijms23010207.
Kiwifruit is moderately sweet and sour and quite popular among consumers; it has been widely planted in some areas of the world. In 2019, the crown gall disease of kiwifruit was discovered in the main kiwifruit-producing area of Guizhou Province, China. This disease can weaken and eventually cause the death of the tree. The phylogeny, morphological and biological characteristics of the bacteria were described, and were related to diseases. The pathogenicity of this species follows the Koch hypothesis, confirming that is the pathogen of crown gall disease of kiwifruit in China. In this study, Loop-mediated isothermal amplification (LAMP) analysis for genome-specific gene sequences was developed for the specific detection of . The detection limit of the LAMP method is 5 × 10 ng/μL, which has high sensitivity. At the same time, the amplified product is stained with SYBR Green I after the reaction is completed, so that the amplification can be detected with the naked eye. LAMP analysis detected the presence of in the roots and soil samples of the infected kiwifruit plant. The proposed LAMP detection technology in this study offers the advantages of ease of operation, visibility of results, rapidity, accuracy and high sensitivity, making it suitable for the early diagnosis of crown gall disease of kiwifruit.
猕猴桃酸甜适中，深受消费者喜爱；它已在世界上一些地区广泛种植。2019 年，在中国猕猴桃主产区贵州省发现了猕猴桃冠瘿病。这种疾病会削弱甚至最终导致树木死亡。本文描述了细菌的系统发育、形态和生物学特性，并与疾病有关。该种的致病性遵循科赫假设，证实了 是中国猕猴桃冠瘿病的病原菌。在这项研究中，开发了针对基因组特异性基因序列的环介导等温扩增（LAMP）分析方法，用于 的特异性检测。LAMP 方法的检测限为 5×10ng/μL，具有很高的灵敏度。同时，反应完成后用 SYBR Green I 染色扩增产物，使扩增可以肉眼检测。LAMP 分析检测到感染猕猴桃植株的根和土壤样本中存在 。本研究提出的 LAMP 检测技术具有操作简单、结果可视化、快速、准确和高灵敏度的优点，适合猕猴桃冠瘿病的早期诊断。
