Proença Diogo Neves, Fasola Emanuele, Lopes Isabel, Morais Paula V
Department of Life Sciences and Centre for Mechanical Engineering, Materials and Processes, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
CESAM and Department of Biology, University of Aveiro, 3810-005 Aveiro, Portugal.
Int J Environ Res Public Health. 2021 Mar 5;18(5):2585. doi: 10.3390/ijerph18052585.
Microorganisms that live in association with amphibian skin can play important roles in protecting their host. Within the scenarios of global change, it is important to understand how environmental disturbances, namely, metal pollution, can affect this microbiota. The aim of this study is to recognize core bacteria in the skin cultivable microbiota of the Perez frog () that are preserved regardless of the environmental conditions in which the frogs live. The characterization of these isolates revealed characteristics that can support their contributions to the ability of frogs to use metal impacted environments. Frog's skin swabs were collected from populations that inhabit a metal-polluted site and three reference (non-metal polluted) sites. Bacterial strains were isolated, identified, and subjected to an acid mine drainage tolerance (AMD) test, collected upstream from a site heavily contaminated with metals, and tested to produce extracellular polymeric substances (exopolysaccharide, EPS). All frog populations had in their cutaneous cultivable microbiota. Significant growth inhibition was observed in all bacterial isolates exposed to 75% of AMD. EPS production was considered a characteristic of several isolates. The data obtained is a preliminary step but crucial to sustain that the cultivable microbiota is a mechanism for protecting frogs against environmental contamination.
与两栖动物皮肤共生的微生物在保护其宿主方面可发挥重要作用。在全球变化的背景下，了解环境干扰因素，即金属污染，如何影响这种微生物群至关重要。本研究的目的是识别佩雷斯蛙（ ）皮肤可培养微生物群中的核心细菌，这些细菌无论青蛙生活在何种环境条件下都能得以保留。对这些分离菌株的特性进行表征后发现，它们的特性有助于青蛙在受金属影响的环境中生存。从栖息于金属污染场地的 个种群以及三个对照（未受金属污染）场地采集了青蛙的皮肤拭子。分离、鉴定细菌菌株，并对其进行酸性矿山废水耐受性（AMD）测试，这些废水取自一个受金属严重污染的场地的上游，并测试其产生胞外聚合物（胞外多糖，EPS）的能力。所有青蛙种群的皮肤可培养微生物群中都有 。在所有暴露于75% AMD的细菌分离物中均观察到显著的生长抑制。EPS的产生被认为是几种分离物的特征。所获得的数据只是初步结果，但对于证明可培养微生物群是青蛙抵御环境污染的一种机制至关重要。
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