Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Department of Forest and Conservation Sciences, Faculty of Forestry, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Int J Mol Sci. 2021 Mar 5;22(5):2601. doi: 10.3390/ijms22052601.
Ginkgo ( L.) is a deciduous tree species with high timber, medicinal, ecological, ornamental, and scientific values, and is widely cultivated worldwide. However, for such an important tree species, the regulatory mechanisms involved in the photosynthesis of developing leaves remain largely unknown. Here, we observed variations in light response curves (LRCs) and photosynthetic parameters (photosynthetic capacity (P) and dark respiration rate (R)) of leaves across different developmental stages. We found the divergence in the abundance of compounds (such as 3-phospho-d-glyceroyl phosphate, sedoheptulose-1,7-bisphosphate, and malate) involved in photosynthetic carbon metabolism. Additionally, a co-expression network was constructed to reveal 242 correlations between transcription factors (TFs) and photosynthesis-related genes ( < 0.05, |r| > 0.8). We found that the genes involved in the photosynthetic light reaction pathway were regulated by multiple TFs, such as bHLH, WRKY, ARF, IDD, and TFIIIA. Our analysis allowed the identification of candidate genes that most likely regulate photosynthesis, primary carbon metabolism, and plant development and as such, provide a theoretical basis for improving the photosynthetic capacity and yield of ginkgo trees.
银杏（Ginkgo）是一种落叶乔木，具有很高的木材、药用、生态、观赏和科学价值，在全球范围内广泛种植。然而，对于这样一个重要的树种，其发育叶片光合作用的调控机制在很大程度上仍然未知。在这里，我们观察了不同发育阶段叶片的光响应曲线（LRC）和光合作用参数（光合能力（P）和暗呼吸速率（R））的变化。我们发现参与光合作用碳代谢的化合物（如 3-磷酸甘油酸、景天庚酮糖-1,7-二磷酸和苹果酸）的丰度存在差异。此外，构建了一个共表达网络，揭示了转录因子（TFs）和光合作用相关基因之间的 242 个相关性（<0.05，|r|>0.8）。我们发现，参与光合作用光反应途径的基因受到多个 TF 的调节，如 bHLH、WRKY、ARF、IDD 和 TFIIIA。我们的分析确定了候选基因，这些基因可能调节光合作用、初级碳代谢和植物发育，从而为提高银杏的光合作用能力和产量提供了理论依据。
