Li Shan, Xia Jingqing, Liu Shouzan, Li Zhe, Shen Qiong, Yang Feng, Liu Xinhong, Bai Yan
College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou, China.
Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Hangzhou, China.
Front Plant Sci. 2024 Oct 23;15:1477280. doi: 10.3389/fpls.2024.1477280. eCollection 2024.
is a folk and rare medicinal plant, and specifically, it is distributed in the south, China. To investigate the cumulative properties of its medicinal components, we examined the effect of UV light on flavonoid content and related enzyme activity changes in .
The leaves and tubers were treated with UV-A, UV-B and UV-C for 1 h, 1L/23D h, 3 h and 3L/21D h (D represents darkness treatment). High-performance liquid chromatography (HPLC) analysis showed that the content of many flavonoids decreased significantly during UV-A treatment, increased after UV-B and UV-C irradiation and accumulated again after darkness treatment.
In the root tubers of the UV-A group, naringin content in the 3L/21D h group (0.069 μg/g) was 16.30 times higher than that of 3 h group (0.0042 μg/g). The rutin content was elevated after UV irradiation but was not detected in the CK group. The test results of the enzyme-linked kit indicated that the activities of many enzymes were higher in the UV-A and UV-B irradiation groups than those in the CK group, but the results were reversed in the UV-C treatment. After darkness treatment, the activities of most enzymes were higher than those with UV irradiation alone; F3'5'H activity in the 3L/21D h group (97.25 U/L) was 1.24 times higher than that in the 3 h group (78.12 U/L) in the UV-A-treated group.
The study results suggest that appropriate UV-B and UV-C irradiation, as well as darkness supplementation, had a promotive effect on flavonoids in the leaves and root tubers of . Additionally, UV irradiation and darkness treatment enhanced the activity of most enzymes.
是一种民间稀有药用植物，具体分布于中国南方。为研究其药用成分的累积特性，我们检测了紫外线对其黄酮类化合物含量及相关酶活性变化的影响。
将叶片和块茎分别用UV-A、UV-B和UV-C处理1小时、1L/23D小时、3小时和3L/21D小时（D表示黑暗处理）。高效液相色谱（HPLC）分析表明，UV-A处理期间许多黄酮类化合物含量显著下降，UV-B和UV-C照射后增加，黑暗处理后再次累积。
UV-A组块根中，3L/21D小时组（0.069μg/g）的柚皮苷含量是3小时组（0.0042μg/g）的16.30倍。UV照射后芦丁含量升高，但对照组未检测到。酶联试剂盒检测结果表明，UV-A和UV-B照射组中许多酶的活性高于对照组，但UV-C处理结果相反。黑暗处理后，大多数酶的活性高于仅UV照射组；UV-A处理组中，3L/21D小时组（97.25U/L）的F3'5'H活性是3小时组（78.12U/L）的1.24倍。
研究结果表明，适当的UV-B和UV-C照射以及黑暗补充对其叶片和块根中的黄酮类化合物有促进作用。此外，UV照射和黑暗处理增强了大多数酶的活性。
