Mashkour Narges, Jones Karina, Wirth Wytamma, Burgess Graham, Ariel Ellen
College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD 4814, Australia.
College of Medicine and Dentistry, James Cook University, Townsville, QLD 4814, Australia.
Animals (Basel). 2021 Mar 5;11(3):697. doi: 10.3390/ani11030697.
Characterised by benign tumours, fibropapillomatosis (FP) is a debilitating disease that predominantly afflicts the endangered green turtle (). A growing body of histological and molecular evidence has associated FP tumours with Chelonid alphaherpesvirus 5 (ChHV5). However, a recent study which detected both ChHV5 and papillomavirus 1 (CmPV1) DNA in FP tumour tissues has challenged this hypothesis. The present study aimed to establish a probe-based qPCR to assess the wider prevalence of CmPV1 and co-occurrence with ChHV5 in 275 marine turtles foraging in waters adjacent to the east coast of Queensland, Australia: three categories: Group A (FP tumours), Group B (non-tumoured skin from FP turtles) and Group C (non-tumoured skin from turtles without FP). Concurrent detection of ChHV5 and CmPV1 DNA is reported for all three categories, where Group A had the highest rate (43.5%). ChHV5 viral loads in Group A were significantly higher than loads seen in Group B and C. This was not the case for CmPV1 where the loads in Group B were highest, followed by Group A. However, the mean CmPV1 load for Group A samples was not significantly different to the mean load reported from Group B or C samples. Collectively, these results pivot the way we think about FP; as an infectious disease where two separate viruses may be at play.
纤维乳头瘤病（FP）以良性肿瘤为特征，是一种主要折磨濒危绿海龟的使人衰弱的疾病。越来越多的组织学和分子证据将FP肿瘤与海龟α疱疹病毒5（ChHV5）联系起来。然而，最近一项在FP肿瘤组织中同时检测到ChHV5和乳头瘤病毒1（CmPV1）DNA的研究对这一假设提出了挑战。本研究旨在建立一种基于探针的定量聚合酶链反应（qPCR），以评估在澳大利亚昆士兰州东海岸附近水域觅食的275只海龟中CmPV1的广泛流行情况以及与ChHV5的共现情况，分为三类：A组（FP肿瘤）、B组（来自患FP海龟的无肿瘤皮肤）和C组（来自未患FP海龟的无肿瘤皮肤）。报告了所有三类中ChHV5和CmPV1 DNA的同时检测情况，其中A组的检出率最高（43.5%）。A组的ChHV5病毒载量显著高于B组和C组。CmPV1的情况并非如此，B组的病毒载量最高，其次是A组。然而，A组样本的平均CmPV1载量与B组或C组样本报告的平均载量没有显著差异。总体而言，这些结果改变了我们对FP的看法；它是一种可能由两种不同病毒引发的传染病。
