Laboratório de Patologia Comparada de Animais Selvagens, Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Código Endereçamento Postal, 05508-010, São Paulo, São Paulo State, Brazil.
Instituto Federal de São Paulo, Campus Cubatão, Rua Maria Cristina 50, Casqueiro, Código Endereçamento Postal, 11533-160, Cubatão, São Paulo State, Brazil.
J Aquat Anim Health. 2021 Dec;33(4):252-263. doi: 10.1002/aah.10142. Epub 2021 Oct 11.
Chelonid alphaherpesvirus 5 (ChHV5) has been consistently associated with fibropapillomatosis (FP), a neoplastic disease that affects sea turtles globally. The DNA of ChHV5 has been detected in cutaneous and noncutaneous tissues (e.g., lung) of green sea turtles Chelonia mydas with (FP+) and without (FP-) clinical signs of FP, indicating a persistent ChHV5 infection. Previously published and custom primer pairs were used to amplify the fragments of ChHV5 unique long (UL) partial genes (UL30 and UL18) through end-point PCR from cutaneous tumors (n = 31), nontumored skin (n = 49), and lungs (n = 26) from FP+ (n = 31) and FP- (n = 18) green sea turtles. The DNA of ChHV5 was detected in cutaneous tumors (80.6%, 25/31), nontumored skin (74.2%, 23/31 FP+; 27.8%, 5/18 FP-), and lung samples (91.7%, 11/12 FP+; 100%, 14/14 FP-). The high occurrence of ChHV5 observed in lung samples from FP- individuals was unexpected (14/14), providing the first evidence of ChHV5 DNA presence in lungs of individuals without FP. Our results also revealed high ChHV5 occurrence among the tested cohort (93.9%, 46/49) and suggested that a large proportion (83.4%, 15/18) of FP- green sea turtles had subclinical ChHV5 infections. Hence, our findings support the hypothesis that ChHV5 requires one or more possibly environmental or immune-related co-factors to induce FP.
龟α疱疹病毒 5 (ChHV5) 一直与纤维瘤病 (FP) 有关，FP 是一种影响全球海龟的肿瘤性疾病。ChHV5 的 DNA 已在具有 (FP+) 和不具有 (FP-) FP 临床症状的绿海龟 Chelonia mydas 的皮肤和非皮肤组织（例如肺）中检测到，表明存在持续性 ChHV5 感染。先前发表的和定制的引物对被用于通过终点 PCR 从 FP+（n=31）和 FP-（n=18）绿海龟的皮肤肿瘤（n=31）、非肿瘤皮肤（n=49）和肺（n=26）中扩增 ChHV5 独特的长（UL）部分基因（UL30 和 UL18）的片段。在皮肤肿瘤（80.6%，25/31）、非肿瘤皮肤（74.2%，23/31 FP+；27.8%，5/18 FP-）和肺样本（91.7%，11/12 FP+；100%，14/14 FP-）中均检测到 ChHV5 的 DNA。在没有 FP 的个体的肺样本中观察到 ChHV5 的高发生率（14/14）出乎意料，这首次提供了 ChHV5 DNA 存在于无 FP 个体肺中的证据。我们的结果还表明，在所测试的队列中 ChHV5 的发生率很高（93.9%，46/49），并且表明很大一部分（83.4%，15/18）FP-绿海龟存在亚临床 ChHV5 感染。因此，我们的研究结果支持 ChHV5 需要一个或多个可能与环境或免疫相关的共同因素来诱导 FP 的假说。
