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R- 应答蛋白2和WNT/β-连环蛋白信号通路是猪卵泡发育和体外成熟所必需的。

R-Spondin 2 and WNT/CTNNB1 Signaling Pathways Are Required for Porcine Follicle Development and In Vitro Maturation.

作者信息

Hwang Seon-Ung, Yoon Junchul David, Kim Mirae, Cai Lian, Choi Hyerin, Oh Dongjin, Kim Eunhye, Hyun Sang-Hwan

机构信息

Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea.

Institute of Stem Cell & Regenerative Medicine (ISCRM), Chungbuk National University, Cheongju 28644, Korea.

出版信息

Animals (Basel). 2021 Mar 5;11(3):709. doi: 10.3390/ani11030709.

DOI:10.3390/ani11030709
PMID:33807916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7998564/
Abstract

The secretion of oocyte-derived paracrine factors, such as R-spondin2, is an essential mechanism for follicle growth by promoting the proliferation and differentiation of cumulus cells around oocytes. In the present study, we aimed to identify the effect of R-spondin2 during follicular development. First, R-spondin2-related factors (R-spondin2, CTNNB1, LGR4, and LGR5) were identified through immunofluorescence in porcine ovarian tissue. CTNNB1 was expressed in ooplasm, and CTNNB1 and LGR4 were expressed in granulosa cells. In addition, R-spondin2, LGR4, and LGR5 were expressed in the theca interna. These results imply that these proteins play a major role in porcine follicular development. In addition, the effects of R-spondin2 on the in vitro maturation process of porcine cumulus oocyte complexes and subsequent embryonic development were confirmed. A treatment of 100 ng/mL R-spondin2 in the in vitro maturation (IVM) process increased nuclear maturation and increased the expression of mRNA in cumulus cells. The EGFR-ERK signal is essential for oocyte maturation, ovulation, and luteinization. R-spondin2 treatment also increased the expression of CTNNB1 and EGFR in primary cultured cumulus cells. In conclusion, RSPO2 and WNT/CTNNB1 signaling pathways are required for porcine follicle development and are predicted to be involved in the EGFR-ERK signaling pathway.

摘要

卵母细胞衍生的旁分泌因子（如R-spondin2）的分泌是促进卵母细胞周围卵丘细胞增殖和分化从而实现卵泡生长的重要机制。在本研究中，我们旨在确定R-spondin2在卵泡发育过程中的作用。首先，通过免疫荧光法在猪卵巢组织中鉴定了R-spondin2相关因子（R-spondin2、CTNNB1、LGR4和LGR5）。CTNNB1在卵质中表达，CTNNB1和LGR4在颗粒细胞中表达。此外，R-spondin2、LGR4和LGR5在内膜中表达。这些结果表明这些蛋白质在猪卵泡发育中起主要作用。此外，还证实了R-spondin2对猪卵丘卵母细胞复合体体外成熟过程及后续胚胎发育的影响。在体外成熟（IVM）过程中用100 ng/mL的R-spondin2处理可提高核成熟度，并增加卵丘细胞中mRNA的表达。EGFR-ERK信号对于卵母细胞成熟、排卵和黄体化至关重要。R-spondin2处理还增加了原代培养的卵丘细胞中CTNNB1和EGFR的表达。总之，RSPO2和WNT/CTNNB1信号通路是猪卵泡发育所必需的，并且预计参与EGFR-ERK信号通路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/979493742864/animals-11-00709-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/045baf9965c3/animals-11-00709-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/7bc8adb439b3/animals-11-00709-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/2efc5552afd0/animals-11-00709-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/9d315575d0a1/animals-11-00709-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/979493742864/animals-11-00709-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/045baf9965c3/animals-11-00709-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/7bc8adb439b3/animals-11-00709-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/2efc5552afd0/animals-11-00709-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/9d315575d0a1/animals-11-00709-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b2d/7998564/979493742864/animals-11-00709-g005.jpg

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