Yoo Jeong-Ju, Shin Hee Bong, Song Ju Sun, Kim Minjung, Yun Jina, Kim Zisun, Lee Yoo Min, Lee Sang Wook, Lee Kwang Woo, Kim Woong Bin, Ryu Chang Beom, Park Sung-Woo, Park Seong Kyu, Song Ho-Yeon, Kim Young Ho
Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea.
Department of Laboratory Medicine and Genetics, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea.
J Clin Med. 2021 Mar 5;10(5):1097. doi: 10.3390/jcm10051097.
Traditionally, the diagnostic mainstay of recurrent urinary tract infection has been urinary culture. However, the causative uropathogen of recurrent cystitis has not been well established. Urine DNA next-generation sequencing (NGS) can provide additional information on these infections. Herein, we compared urine NGS results and urine cultures in patients with acute uncomplicated cystitis (AUC) and recurrent cystitis (RC), and evaluated the difference in microbiome patterns in the NGS results. Patients who underwent urine culture and NGS due to AUC or RC were retrospectively reviewed. All urine samples were collected via a transurethral catheter and studied utilizing a type of NGS called 16S ribosomal RNA gene amplification and sequencing. The sensitivity of urine NGS was significantly higher than that of conventional urine culture (69.0% vs. 16.7%, < 0.05). The detection rate of urine NGS was slightly lower in the RC group than in the AUC group (67.7% vs. 72.7%). Microbiome diversity was significantly higher in the RC group compared to the AUC group ( = 0.007), and the microbiome composition was significantly different between the AUC and RC groups. In the urine NGS results, , and Enterobacteriaceae were found in the AUC group, and , and spp. were detected in the RC group. Urine NGS can significantly increase the diagnostic sensitivity compared to traditional urine culture methods, especially in RC patients. AUC and RC patients had significant differences in bacterial diversity and patterns. Therefore, recurrent cystitis might be approached from a different perspective.
传统上，复发性尿路感染的主要诊断方法是尿液培养。然而，复发性膀胱炎的致病尿路病原体尚未完全明确。尿液DNA下一代测序（NGS）可以为这些感染提供更多信息。在此，我们比较了急性单纯性膀胱炎（AUC）和复发性膀胱炎（RC）患者的尿液NGS结果和尿液培养结果，并评估了NGS结果中微生物群模式的差异。对因AUC或RC接受尿液培养和NGS的患者进行回顾性分析。所有尿液样本均通过经尿道导管采集，并采用一种名为16S核糖体RNA基因扩增和测序的NGS方法进行研究。尿液NGS的敏感性显著高于传统尿液培养（69.0%对16.7%，<0.05）。RC组尿液NGS的检测率略低于AUC组（67.7%对72.7%）。与AUC组相比，RC组的微生物群多样性显著更高（=0.007），且AUC组和RC组之间的微生物群组成存在显著差异。在尿液NGS结果中，AUC组发现了大肠埃希菌、肺炎克雷伯菌和肠杆菌科，RC组检测到了奇异变形杆菌、腐生葡萄球菌和粪肠球菌属。与传统尿液培养方法相比，尿液NGS可以显著提高诊断敏感性，尤其是在RC患者中。AUC和RC患者在细菌多样性和模式上存在显著差异。因此，复发性膀胱炎可能需要从不同角度进行研究。
