Nguyen Thi Truc Linh, Nguyen Thanh Tuu, Tran Thi Hong To, Pham Van Day, Vo Thi Tuyet Minh, Nguyen Trong Nghia, Phan Cong Minh, Cao Ngoc Diep, Truong Quoc Phu
Tra Vinh University, 126, Nguyen Thien Thanh, Tra Vinh 87000, Vietnam.
Aquaculture Pharmacy Company Limited, 149/41, Hoang Van Thu Street, An Cu Ward, Ninh Kieu District, Can Tho 94000, Vietnam.
Biology (Basel). 2021 Mar 30;10(4):280. doi: 10.3390/biology10040280.
This study aimed to evaluate the growth, survival rate, and resistance to acute hepatopancreatic necrosis disease (AHPND) of white leg shrimp () by using , , and mixed with feed, and at the same time supplying CNP in a ratio of 15:1:0.1 to the water. As a result, the treatments that shrimp were fed with feed containing lactic acid bacteria (LAB), especially , have increased shrimp growth, total hemocyte cells, granulocyte cells, and hyaline cells significantly ( < 0.05) in comparison to the control group. The supply of CNP to the water has promoted the intensity of effects on shrimp health and significantly decreased total hemocyte cells, granulocyte cells, and hyaline cells by 30-50% in the period after three days of the challenge, except in treatment, which had only a 20% decrease compared to other treatments. In CNP supplying treatments, the AHPND infected rate and mortality of shrimp were higher than those in other treatments. In summary, the supply of CNP had significantly reduced the shrimp's immune response and promoted the susceptibility of shrimp to AHPND in both cases of use with and without LAB-containing diets.
本研究旨在通过使用嗜酸乳杆菌、植物乳杆菌和干酪乳杆菌与饲料混合，并同时以15:1:0.1的比例向水中供应CNP，来评估凡纳滨对虾的生长、存活率以及对急性肝胰腺坏死病（AHPND）的抗性。结果表明，与对照组相比，用含有乳酸菌（LAB）的饲料，特别是嗜酸乳杆菌喂养凡纳滨对虾的处理组，显著提高了凡纳滨对虾的生长、总血细胞、粒细胞和透明细胞数量（P<0.05）。向水中供应CNP促进了其对凡纳滨对虾健康的影响强度，并且在攻毒三天后的时间段内，除了嗜酸乳杆菌处理组相比其他处理组仅降低了20%外，总血细胞、粒细胞和透明细胞数量显著降低了30%-50%。在供应CNP的处理组中，凡纳滨对虾的AHPND感染率和死亡率高于其他处理组。总之，无论在使用含LAB饲料还是不含LAB饲料的情况下，供应CNP均显著降低了凡纳滨对虾的免疫反应，并提高了凡纳滨对虾对AHPND的易感性。
