Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Fish Shellfish Immunol. 2018 Jul;78:42-51. doi: 10.1016/j.fsi.2018.04.010. Epub 2018 Apr 4.
To study the effects of probiotic-added food on the survival and growth of abalone (Haliotis discus hannai Ino), the expression levels of nonspecific immune genes and the anti-Vibrio parahaemolyticus infection were examined. During an 8-week culturing experiment in an indoor aquarium and a 2-week V. parahaemolyticus artificial infection experiment, the control group was fed with untreated food once a day, while the experimental groups (L1, L2 and L3) were fed with Lactobacillus pentosus added food. The concentration of probiotics in the experimental food was 10 cfu/g (L1), 10 cfu/g (L2) and 10 cfu/g (L3), respectively. The results showed that the survival rate, shell length-specific growth rate, and the food conversion rate (FCR) of abalones in L1 and L2 were significantly higher than the control group. The food intake of abalones in L3 was significantly lower than that in L1, L2 and the control group, but there was no significant difference in FCR identified between L1, L2 and L3. In the L. pentosus-added groups, the total number of blood lymphocytes, lysozyme activity, acid phosphatase, superoxide dismutase, and expression levels of Mn-superoxide dismutase (Mn-SOD) and thioredoxin peroxidase (TP) were significantly higher than the control group, while the malondialdehyde (MDA) content was significantly lower than the control group. The phagocytic activity of blood lymphocytes, catalase activity and the expression levels of heat shock protein 70 (HSP70) of abalones in the control group were significantly lower than that in L1 and L2, but there was no significant difference when compared with L3. The levels of O, NO produced by respiratory burst of blood lymphocytes and the expression levels of catalase (CAT) in L1 and L2 were significantly higher than both L3 and the control group. Seven days after infection with V. parahaemolyticus, all abalones in the control group were dead. After 14 days the cumulative mortality rate of abalones in the L. pentosus-added groups was significantly lower than that in the control group. Therefore, the 10 cfu/g and 10 cfu/g L. pentosus-added food not only promoted food intake and growth of abalones, but also improved their non-specific immunity and reduced V. parahaemolyticus infection, indicating that this strain is a good potential candidate for probiotic added food in the aquaculture industry.
为了研究益生菌添加食物对鲍鱼（Haliotis discus hannai Ino）存活和生长的影响，研究了非特异性免疫基因的表达水平和抗副溶血性弧菌感染的能力。在室内水族箱中进行了 8 周的培养实验和 2 周的副溶血性弧菌人工感染实验中，对照组每天投喂未处理的食物，而实验组（L1、L2 和 L3）则投喂添加了戊糖片球菌的食物。实验食物中益生菌的浓度分别为 10 cfu/g（L1）、10 cfu/g（L2）和 10 cfu/g（L3）。结果表明，L1 和 L2 组鲍鱼的存活率、壳长特定生长率和饲料转化率（FCR）均显著高于对照组。L3 组鲍鱼的摄食量显著低于 L1、L2 和对照组，但 L1、L2 和 L3 之间的 FCR 无显著差异。在添加戊糖片球菌的组中，血液淋巴细胞总数、溶菌酶活性、酸性磷酸酶、超氧化物歧化酶以及 Mn-超氧化物歧化酶（Mn-SOD）和硫氧还蛋白过氧化物酶（TP）的表达水平均显著高于对照组，而丙二醛（MDA）含量显著低于对照组。对照组鲍鱼的血液淋巴细胞吞噬活性、过氧化氢酶活性和热休克蛋白 70（HSP70）的表达水平显著低于 L1 和 L2，但与 L3 无显著差异。L1 和 L2 组血液淋巴细胞呼吸爆发产生的 O2、NO 水平和过氧化氢酶（CAT）的表达水平均显著高于 L3 和对照组。感染副溶血性弧菌 7 天后，对照组所有鲍鱼均死亡。14 天后，添加戊糖片球菌组的鲍鱼累计死亡率明显低于对照组。因此，添加 10 cfu/g 和 10 cfu/g 戊糖片球菌的食物不仅促进了鲍鱼的摄食和生长，还提高了其非特异性免疫力，降低了副溶血性弧菌感染，表明该菌株是水产养殖中益生菌添加食物的良好候选菌株。
