Pałka Krzysztof, Miazga-Karska Małgorzata, Pawłat Joanna, Kleczewska Joanna, Przekora Agata
Faculty of Mechanical Engineering, Lublin University of Technology, Nadbystrzycka 36, 20-618 Lublin, Poland.
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, Chodźki 1, 20-093 Lublin, Poland.
Materials (Basel). 2021 Mar 30;14(7):1704. doi: 10.3390/ma14071704.
The aim of this study was to evaluate the effect of modification with liquid rubber on the adhesion to tooth tissues (enamel, dentin), wettability and ability to inhibit bacterial biofilm formation of resin-based dental composites. Two commercial composites (Flow-Art-flow type with 60% ceramic filler and Boston-packable type with 78% ceramic filler; both from Arkona Laboratorium Farmakologii Stomatologicznej, Nasutów, Poland) were modified by addition of 5% by weight (of resin) of a liquid methacrylate-terminated polybutadiene. Results showed that modification of the flow type composite significantly ( < 0.05) increased the shear bond strength values by 17% for enamel and by 33% for dentine. Addition of liquid rubber significantly ( < 0.05) reduced also hydrophilicity of the dental materials since the water contact angle was increased from 81-83° to 87-89°. Interestingly, modified packable type material showed improved antibiofilm activity against and (quantitative assay with crystal violet), but also cytotoxicity against eukaryotic cells since cell viability was reduced to 37% as proven in a direct-contact WST-8 test. Introduction of the same modification to the flow type material significantly improved its antibiofilm properties (biofilm reduction by approximately 6% compared to the unmodified material, < 0.05) without cytotoxic effects against human fibroblasts (cell viability near 100%). Thus, modified flow type composite may be considered as a candidate to be used as restorative material since it exhibits both nontoxicity and antibiofilm properties.
本研究的目的是评估用液体橡胶改性对树脂基牙科复合材料与牙齿组织（牙釉质、牙本质）的粘附性、润湿性以及抑制细菌生物膜形成能力的影响。两种市售复合材料（含60%陶瓷填料的流动型Flow - Art和含78%陶瓷填料的可填充型Boston；均来自波兰纳苏托夫的Arkona Laboratorium Farmakologii Stomatologicznej）通过添加5%（按树脂重量计）的甲基丙烯酸酯封端的聚丁二烯进行改性。结果表明，流动型复合材料的改性显著（<0.05）提高了剪切粘结强度值，牙釉质提高了17%，牙本质提高了33%。液体橡胶的添加也显著（<0.05）降低了牙科材料的亲水性，因为水接触角从81 - 83°增加到了87 - 89°。有趣的是，改性可填充型材料对变形链球菌和远缘链球菌显示出改善的抗生物膜活性（用结晶紫进行定量测定），但对真核细胞也有细胞毒性，因为在直接接触WST - 8试验中证明细胞活力降低到了37%。对流动型材料进行相同的改性显著改善了其抗生物膜性能（与未改性材料相比，生物膜减少约6%，<0.05），且对人成纤维细胞无细胞毒性作用（细胞活力接近100%）。因此，改性流动型复合材料可被视为一种修复材料的候选材料，因为它兼具无毒性和抗生物膜性能。
