Majumder Mrinmoyee, Palanisamy Viswanathan
Department of Biochemistry and Molecular Biology, College of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.
Methods Protoc. 2021 Mar 19;4(1):22. doi: 10.3390/mps4010022.
Control of gene expression is critical in shaping the pro-and eukaryotic organisms' genotype and phenotype. The gene expression regulatory pathways solely rely on protein-protein and protein-nucleic acid interactions, which determine the fate of the nucleic acids. RNA-protein interactions play a significant role in co- and post-transcriptional regulation to control gene expression. RNA-binding proteins (RBPs) are a diverse group of macromolecules that bind to RNA and play an essential role in RNA biology by regulating pre-mRNA processing, maturation, nuclear transport, stability, and translation. Hence, the studies aimed at investigating RNA-protein interactions are essential to advance our knowledge in gene expression patterns associated with health and disease. Here we discuss the long-established and current technologies that are widely used to study RNA-protein interactions in vivo. We also present the advantages and disadvantages of each method discussed in the review.
基因表达的调控对于塑造原核生物和真核生物的基因型和表型至关重要。基因表达调控途径完全依赖于蛋白质-蛋白质和蛋白质-核酸相互作用，这些相互作用决定了核酸的命运。RNA-蛋白质相互作用在转录和转录后调控中发挥重要作用，以控制基因表达。RNA结合蛋白（RBP）是一类多样的大分子，它们与RNA结合，并通过调节前体mRNA的加工、成熟、核运输、稳定性和翻译在RNA生物学中发挥重要作用。因此，旨在研究RNA-蛋白质相互作用的研究对于推进我们对与健康和疾病相关的基因表达模式的认识至关重要。在这里，我们讨论了长期以来以及目前广泛用于体内研究RNA-蛋白质相互作用的技术。我们还介绍了本综述中讨论的每种方法的优缺点。
