Department of Plant and Microbial Biology, University of Zurich, 8008 Zurich, Switzerland.
Zurich-Basel Plant Science Center, 8092 Zurich, Switzerland.
Cells. 2021 Mar 19;10(3):685. doi: 10.3390/cells10030685.
Plant cells are encapsulated by cell walls whose properties largely determine cell growth. We have previously identified the mutant, which shows defects in seedling root and shoot development. is affected in the () and shows alterations in the structures of Rhamnogalacturonan I (RG I) and RG II, two rhamnose-containing pectins. The data presented here shows that root tissue of the mutant fails to properly differentiate the cell wall in cell corners and accumulates excessive amounts of callose, both of which likely alter the physical properties of cells. A () mutant was identified that alleviates the cell growth defects in . The cell wall differentiation defect is re-established in the mutant and callose accumulation is reduced compared to . The mutation is an allele of the (), which encodes a component of the mediator complex that influences processes central to plant growth and development. Together, the identification of the mutant suggests that changes in cell wall composition and turnover in the mutant have a significant impact on cell growth and reveals a function of CDK8 in cell wall architecture and composition.
植物细胞被细胞壁所包裹，其特性在很大程度上决定了细胞的生长。我们之前已经鉴定出了 突变体，该突变体在幼苗根和芽的发育中表现出缺陷。 突变体在 ()中受到影响，并表现出鼠李半乳糖醛酸聚糖 I (RG I)和 RG II 的结构改变，这两种果胶都含有鼠李糖。这里呈现的数据表明， 突变体的根组织无法在细胞角正确区分细胞壁，并积累了过多的胼胝质，这两者都可能改变细胞的物理特性。鉴定出一个 ()突变体可以缓解 中的细胞生长缺陷。在 突变体中，细胞壁分化缺陷得以重建，与 相比，胼胝质的积累减少。 突变是 ()的等位基因，它编码一个影响植物生长和发育的核心过程的中介复合物的组成部分。总之， 突变体的鉴定表明，细胞壁组成和周转的变化对细胞生长有显著影响，并揭示了 CDK8 在细胞壁结构和组成中的功能。
