Lux P, Breidenbach W, Firrell J
Louisville Institute for Hand and Microsurgery, Jewish Hospital, Ky.
Plast Reconstr Surg. 1988 Jul;82(1):133-44.
The time course of revascularization of nonvascularized and vascularized nerve grafts was followed histologically and quantitatively using microspheres in the saphenous nerve of dogs. Nonvascularized and vascularized nerve grafts were created in opposite hindlimbs of each dog. The intravascular presence of India ink was not observed until the third day postoperatively in the nonvascularized grafts, while it was demonstrated in vascularized grafts on the first postoperative day. Control blood flow in undisturbed nerves ranged from 0.06 to 0.16 ml/min/gm dry weight. On postoperative day 1, flow was not detectable in any of the nonvascularized grafts, while vascularized grafts all had increased flow with a mean of 0.73 ml/min/gm dry weight. By day 3, two of six dogs had some measurable flow in the nonvascularized grafts, while flow in vascularized grafts had reached a mean of 1.88 ml/min/gm dry weight. By day 6, blood flow rate had increased to 7.63 and 4.72 ml/min/gm dry weight for nonvascularized and vascularized grafts, respectively. By this avoidance of up to 3 days of warm ischemia, there may be some advantage to vascularized nerve grafts even in a well-vascularized bed.
在犬的隐神经中，使用微球对非血管化和血管化神经移植物的血管再生时间进程进行了组织学和定量研究。在每只犬的对侧后肢分别制作非血管化和血管化神经移植物。非血管化移植物在术后第三天血管内才出现印度墨汁，而血管化移植物在术后第一天就有印度墨汁显示。未受干扰的神经的对照血流量为0.06至0.16毫升/分钟/克干重。术后第一天，所有非血管化移植物均未检测到血流，而血管化移植物的血流均增加，平均为0.73毫升/分钟/克干重。到第三天，六只犬中有两只的非血管化移植物有一些可测量的血流，而血管化移植物的血流平均达到1.88毫升/分钟/克干重。到第六天，非血管化和血管化移植物的血流速度分别增加到7.63和4.72毫升/分钟/克干重。通过避免长达3天的热缺血，即使在血管丰富的床中，血管化神经移植物可能也有一些优势。
