Paulovičová Ema, Kroneková Zuzana, Paulovičová Lucia, Majerčíková Monika, Kronek Juraj
Immunol & Cell Culture Laboratories, Department Immunochemistry of Glycoconjugates, Center of Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovakia.
Department for Biomaterials Research, Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.
Materials (Basel). 2021 Mar 12;14(6):1371. doi: 10.3390/ma14061371.
Poly(2-isopropenyl-2-oxazoline) (PIPOx) represents a functional polymer with high potential for drug delivery, tissue engineering, and immunomodulation. The immunomodulatory efficiency of the PIPOx formulation has been studied in vitro following splenic cells and RAW 264.7 macrophages exposition. The cell-specific immunomodulative effect on production of Th1, Th2, Th17, and Treg signature cytokines has been demonstrated. The impact on the functionality of PIPOx-sensitized RAW 264.7 macrophages was assessed by cell phagocytosis. Time- and concentration-dependent cell internalization and intracellular organelles colocalization of fluorescently labeled PIPOx has been examined. The in vitro results demonstrated the PIPOx bioavailability and the capability of triggering immune cell responses resulting in the induced production of cell-specific signature interleukins, important prerequisite properties for future potential biomedical applications.
聚（2-异丙烯基-2-恶唑啉）（PIPOx）是一种具有高潜力的功能聚合物，可用于药物递送、组织工程和免疫调节。在脾细胞和RAW 264.7巨噬细胞暴露后，已在体外研究了PIPOx制剂的免疫调节效率。已证明对Th1、Th2、Th17和Treg标志性细胞因子产生具有细胞特异性免疫调节作用。通过细胞吞噬作用评估了PIPOx致敏的RAW 264.7巨噬细胞功能的影响。已检查了荧光标记的PIPOx的时间和浓度依赖性细胞内化以及细胞内细胞器共定位。体外结果证明了PIPOx的生物利用度以及触发免疫细胞反应的能力，从而导致诱导产生细胞特异性标志性白细胞介素，这是未来潜在生物医学应用的重要前提特性。
