Department for Biomaterials Research, Polymer Institute of the Slovak Academy of Sciences, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.
Department of Glycomaterials, Immunology & Cell Culture Laboratories, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 84538 Bratislava, Slovakia.
Biomacromolecules. 2024 Jun 10;25(6):3288-3301. doi: 10.1021/acs.biomac.3c01434. Epub 2024 May 28.
Poly(2-isopropenyl-2-oxazoline) (PIPOx) represents a universal polymer platform with pendant 2-oxazoline groups, allowing the preparation of biomaterials for various biomedical applications. However, there is a lack of information on PIPOx concerning the effect of molar mass () on cytotoxicity and bioimmunological properties. Here, aqueous copper(0)-mediated reversible-deactivation radical polymerization (Cu-RDPR) was used for the preparation of PIPOx with defined and low dispersity. PIPOx of different are used for the synthesis of conjugates with ibuprofen (5 mol %), the nonsteroidal anti-inflammatory drug. The release of ibuprofen at 37 °C and different pH values is monitored using high-performance liquid chromatography, where the rate of drug release increases with increasing pH and lower . In vitro cytotoxicity and bioimmunological properties of PIPOx and drug conjugates are studied using 3D reconstructed tissue models of the human epidermis and intestinal epithelium. We demonstrate low cytotoxicity of PIPOx and conjugates with different values on both 3D tissue models.
聚（2-异丙基-2-恶唑啉）（PIPOx）是一种具有 2-恶唑啉侧基的通用聚合物平台，可用于制备各种生物医学应用的生物材料。然而，关于 PIPOx 的摩尔质量（）对细胞毒性和生物免疫特性的影响的信息还很缺乏。在此，采用铜（0）介导的可控/活性自由基聚合（Cu-RDPR）制备了具有确定摩尔质量和低分散性的 PIPOx。不同摩尔质量的 PIPOx 用于合成 5mol%布洛芬（非甾体抗炎药）的缀合物。使用高效液相色谱法监测 37°C 和不同 pH 值下布洛芬的释放，药物释放率随 pH 值升高和摩尔质量降低而增加。使用人表皮和肠道上皮的 3D 重建组织模型研究 PIPOx 和药物缀合物的体外细胞毒性和生物免疫特性。我们证明了具有不同摩尔质量值的 PIPOx 和缀合物在两种 3D 组织模型上的低细胞毒性。
